pRSET A, B, and C

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pRSET A, B, and C

Version D

111301

25-0213

pRSET A, B, and C

For high-level expression of recombinant proteinsin

E. coli

Catalog no. V351-20

www.invitrogen.comtech_service@invitrogen.com

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iii

Table of Contents

Table of Contents............................................................................................................................iii

Kit Contents and Storage.................................................................................................................iv

Accessory Products..........................................................................................................................v

Introduction....................................................................................................................1

Overview..........................................................................................................................................1

Methods..........................................................................................................................3

General Cloning................................................................................................................................3

Cloning into pRSET A, B, and C.....................................................................................................4

Expression........................................................................................................................................8

Purification.....................................................................................................................................11

Appendix......................................................................................................................12

Recipes...........................................................................................................................................12

Map of pRSET A, B, and C............................................................................................................14

Features of pRSET A, B, and C......................................................................................................15

Z.......................................................................................................................16

Transformation Protocol for TOP10F

and BL21(DE3)pLysS.......................................................17

Technical Service...........................................................................................................................18

Purchaser Notification....................................................................................................................20

Product Qualification and References............................................................................................21

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