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Table Of Contents

Introduction to High Performance Liquid Chromatography
Historical Aspects
Separation Process
Typical Column Packing Support
Typical Column Supports – Styrene DVB
Typical Column Supports -Styrene DVB
Modes of Liquid Chromatography
Differences in Gas and Liquid Chromatography
Instrumentation
Chromatographic Parameters
Separation Parameters
Review Questions
Review Questions 1A
The Separation Process
In This Section, You Will Learn
The Goal of Separation
Tools for Achieving a Separation
Resolution Values
Calculate a Resolution Value
Factors Influencing Resolution
Resolution Factors
Separation Selectivity
Effect of Temperature on Separation
Capacity Factor k’
Capacity Factor k’
Capacity Factor
Useful Solvents for Reversed-Phase Chromatography
Useful Solvents for Reversed-Phase Chromatography
Solvent Strength: Normal Phase
Solvent Strength -Normal Phase
Normal Phase: Solvent Strength
Normal Phase -Solvent Strength
Efficiency
Calculating Efficiency
Dispersion: Eddy Diffusion
Dispersion -Eddy Diffusion
Dispersion: Longitudinal Diffusion
Dispersion -Longitudinal Diffusion
Dispersion: Mass Transfer
Dispersion -Mass Transfer
Dispersion
Typical Flow Rates
Extra-Column Band Broadening
Increasing Resolution
Peak Symmetry
Worksheets
Mobile Phase Composition -Gradient Elution
Gradient Development
Practical Considerations for Gradient Elution
Practical Aspects of Performing Analyses
Mobile Phase Preparation Filtration
Mobile Phase Degassing
Vacuum Degassing
Solvent Miscibility
Mobile Phase UV Cut-Off
Column Care
Pre-Columns and Guard Columns
Pre-columns and Guard Columns
Syringe Wash: HP 1090
Priming
Column Equilibration
Preparing Samples: Filtering
Preparing Samples -Filtering
Preparing Samples
Worksheet
HPLC Instrumentation
In This Section, You Will Learn:
HPLC Tubing
Fittings
Filters
Functions of the SDS
Multichannel Gradient Valve
Multi-channel Gradient Valve
Dual Piston Parallel Pump
Dual Piston Series Pump
Ball Valves
Metering Pump Seals and Pistons
Diaphragm Pump
Sieves and Filters
Damping Unit
1090 SDS
Quaternary Pump
Manual Injection
Auto-injection System
Auto-Injection Systems
Rotor Seals
Fluorescence Detection
Refractive Index Detection
Refractive Index Detectors
Light Scattering Detection
Light Scattering Detector
Electrochemical Detection
Electrochemical Detectors
Conductivity Detection
Radiometric Detectors
HPLC Troubleshooting
In This Section You Will Learn:
Record Keeping
Proper Care of the HPLC
Peak Retention Time and Precision
Peak Retention Time and Area Precision
Common Pump Problems
Pressure Problems
Pressure Problem
Baseline Fluctuations
Noisy Baseline
Mixing Problems
Manual Injection Valve
Auto-Injectors
Good Column Practices
Column Frit Replacement
Column Regeneration
Detector Performance
Detector Time Constant
Detector: Time Constant
Detector Heat Exchangers
Detectors: Heat Exchangers
Noisy Baselines
Drifting Baselines
Ghost Peaks
Extra-Column Dispersion
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HPLC Complete Theory

HPLC Complete Theory

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Categories:Types, Research, Science
Published by: Muhammad Sohail Anwar on Jul 24, 2012
Copyright:Attribution Non-commercial

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01/23/2013

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