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ABSTRACTTitle of Thesis:ADAPTATION OFA/MALLARD/POTSDAM/178-4/83 (H2N2)IN JAPANESE QUAIL LEADS TOREPLICATION AND TRANSMISSION INCHICKENSErin Maureen Sorrell, Master of Science, 2005Thesis Directed By:Assistant Professor Daniel R. PerezDepartment of Veterinary MedicineVA-MD Regional College of VeterinaryMedicine and The University of Maryland,College Park Influenza is a single stranded, negative-sense RNA virus with a segmentedgenome that can infect avian and mammalian species. Influenza viruses from theavian reservoir do not seem to replicate efficiently in humans upon directtransmission. Therefore, an intermediate host is involved in generating mutations tocreate a more transmissible or an avian-human reassortant virus.Quail have been highlighted as a potential reservoir and intermediate host of avian influenza. To assess the potential of quail as an intermediate host,A/Mallard/Postdam/178-4/83, H2N2 was tested to determine if through adaptation inquail a mallard strain can replicate and transmit in quail, as well as other avianspecies. After six serial passages of lung homogenate a virus arose, which replicatedand transmitted directly to contact quail. When chickens were infected with this
 
quail-adapted virus replication and transmission were observed, while no replicationwas noted in the chickens infected with wild type H2N2 virus.
 
ADAPTATION OF A/MALLARD/POTSDAM/178-4/83 (H2N2)IN JAPANESE QUAIL LEADS TO REPLICATION AND TRANSMISSION INCHICKENSByErin Maureen SorrellThesis submitted to the Faculty of the Graduate School of theUniversity of Maryland, College Park, in partial fulfillmentof the requirements for the degree of Master of Science2005Advisory Committee:Assistant Professor Daniel Perez Chair/ Advisor Associate Professor Jeffrey DeStefanoProfessor Mary Ann Ottinger Professor Siba SamalAssistant Professor Xiaoping Zhu
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