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As 5013.10-2009 Food Microbiology Microbiology of Food and Animal Feeding Stuffs - Horizontal Method for The

As 5013.10-2009 Food Microbiology Microbiology of Food and Animal Feeding Stuffs - Horizontal Method for The

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08/20/2013

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 AS 5013.10—2009
 
 www.standards.org.au
Australian Standard
®
 
Food microbiologyMethod 10: Microbiology of food and animalfeeding stuffs—Horizontal method for thedetection of 
Salmonella
 
spp. (ISO 6579:2002,MOD)
PREFACE
This Standard was prepared by the Standards Australia Committee FT-024, Food Productsand Subcommittee FT-024-01, Food Microbiology, to supersede AS 5013.10—2004.This Standard is an adoption with national modifications and has been reproduced fromISO 6579:2002,
 Microbiology of food and animal feeding stuffs—Horizontal method fordetection of 
 
Salmonella
 
spp
. and its Corrigendum 1, ISO 6579:2002/Cor.1:2004, and itsAmendment 1, ISO 6579:2002/Amd.1:2007, which are added after the main source text.The Corrigendum corrects Clause B.3.1.2; the pH value is changed from 8.2
±
0.2 to8.0
±
0.2 at 25°C. Amendment 1 adds a new Annex D on detection of 
Salmonella
spp. inanimal faeces.The objective of this revision is to incorporate ISO Amendment 1 and Australian technical variations to the following Clauses of ISO 6579:(a)
 
Clause 9, Procedure.(b)
 
Clause 9.5.2, Selection of colonies for confirmation.(c)
 
Clause 9.5.4, Serological confirmation and serotyping.(d)
 
Clause 9.5.6, Definitive confirmation.These variations which are necessary for Australian conditions are listed in Appendix ZZ,which is added at the end of the source text.The additional requirements on confirmation are included to increase the precision of thetest results.As this Standard is reproduced from an International Standard, the following applies:(i) Its number appears on the cover and title page, while the International Standardnumber appears only on the cover.(ii) In the source text ‘this International Standard’ should read ‘this Australian Standard’.(iii) A full point substitutes for a comma when referring to a decimal marker.(iv) Substitute ‘mL’ for ‘ml’ wherever it appears.
A S  5  0  3 . 0  0  0  9 
i
   T   h   i  s   i  s  a   f  r  e  e   5  p  a  g  e  s  a  m  p   l  e .   A  c  c  e  s  s   t   h  e   f  u   l   l  v  e  r  s   i  o  n  a   t   h   t   t  p  :   /   /   i  n   f  o  s   t  o  r  e .  s  a   i  g   l  o   b  a   l .  c  o  m .
 
 
References to International Standards should be replaced by references to AustralianStandards, as follows:
 Reference to International Standard Australian Standar
ISO AS68876887-1Microbiology of food and animalfeeding stuffs—Preparation of test samples, initial suspensionand decimal dilutions formicrobiological examinationPart 1: General rules for thepreparation of the initialsuspension and decimal dilutions50135013.11.1Food microbiologyMethod 11.1: Microbiology of food and animal feeding stuffs— Preparation of test samples, initialsuspension and decimal dilutionsfor microbiological examination— General rules for the preparationof the initial suspension anddecimal dilutions7218 Microbiology of food and animalfeeding stuffs—General rules formicrobiological examinations5013.14 Method 14: Microbiology of foodand animal feeding stuffs— General rules for microbiologicalexaminations8261 Milk and milk products—Generalguidance for the preparation of test samples, initial suspensionsand decimal dilutions formicrobiological examination5013.17 Method 17: Milk and milkproducts—General guidance forthe preparation of test samples,initial suspensions and decimaldilutions for microbiologicalexaminationOther international references have not been adopted as Australian Standards.For testing of certain dairy products, alternative enrichment media and selective media aredescribed in ISO 6785:2001,
 Milk and milk products—Detection of Salmonella
.The laboratory should have a clearly defined quality control system to ensure that theapparatus, culture media, reagents and technique are suitable for the test. The use of positive controls is part of this system.The terms ‘normative’ and ‘informative’ have been used in this Standard to define theapplication of the annex or appendix to which they apply. A ‘normative’ annex or appendixis an integral part of a Standard, whereas an ‘informative’ annex or appendix is only forinformation and guidance.
ii
   T   h   i  s   i  s  a   f  r  e  e   5  p  a  g  e  s  a  m  p   l  e .   A  c  c  e  s  s   t   h  e   f  u   l   l  v  e  r  s   i  o  n  a   t   h   t   t  p  :   /   /   i  n   f  o  s   t  o  r  e .  s  a   i  g   l  o   b  a   l .  c  o  m .
 
 
INTRODUCTION
iii
 
Because of the large variety of food and feed products, this horizontal method may not be appropriate in everydetail for certain products. In this case, different methods, which are specific to these products, may be used if absolutely necessary for justified technical reasons. Nevertheless, every attempt should be made to apply thishorizontal method as far as possible.When this International Standard is next reviewed, account will be taken of all information then available regardingthe extent to which this horizontal method has been followed and the reasons for deviations from this method in thecase of particular products.The harmonization of test methods cannot be immediate, and for certain groups of products InternationalStandards and/or national standards may already exist that do not comply with this horizontal method. It is hopedthat when such standards are reviewed they will be changed to comply with this International Standard so thateventually the only remaining departures from this horizontal method will be those necessary for well-establishedtechnical reasons.
   T   h   i  s   i  s  a   f  r  e  e   5  p  a  g  e  s  a  m  p   l  e .   A  c  c  e  s  s   t   h  e   f  u   l   l  v  e  r  s   i  o  n  a   t   h   t   t  p  :   /   /   i  n   f  o  s   t  o  r  e .  s  a   i  g   l  o   b  a   l .  c  o  m .

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