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r05312304 Genetic Engineering

r05312304 Genetic Engineering

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Published by Srinivasa Rao G

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Published by: Srinivasa Rao G on Jan 18, 2008
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09/12/2008

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Code No: R05312304
Set No. 1
III B.Tech I Semester Regular Examinations, November 2007GENETIC ENGINEERING(Bio-Technology)Time: 3 hours Max Marks: 80Answer any FIVE QuestionsAll Questions carry equal marks
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1. Differentiate between:(a) Basal level transcription and induction(b) CAP and CRP. [8
×
2]2. What are reporter genes? How are these useful in analyzing gene function? [16]3. How are plasmids isolated from bacteria? Explain. [16]4. Comment on:(a) Taq polymerase(b) Alkaline phosphatase(c) Polynucleotide phosphorylase(d) Klenow polymerase. [4
×
4]5. What is the basis for immunological methods used in screening of recombinantclones? [16]6. How does one identify a PCR product? In a particular PCR amplification ex-periment, the product obtained was shorter than the expected length. Give yourcomments on the possible reasons. [16]7. What are the different types of Molecular markers used currently for diagno-sis/identication purposes? [16]8. What are second generation recombinant insulins? Explain how they are differentfrom the native molecule. [16]
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Code No: R05312304
Set No. 2
III B.Tech I Semester Regular Examinations, November 2007GENETIC ENGINEERING(Bio-Technology)Time: 3 hours Max Marks: 80Answer any FIVE QuestionsAll Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. In which operon do you find the leader sequence? How does the translation of leader sequence help in transcription control in certain operons? [16]2. Explain the following terms:(a) Regulatory sequences(b) Enhancers(c) reporter genes(d) gene silencing. [4
×
4]3. Comment on:(a) LTR retroelements(b) non LTR retroelements. [8
×
2]4. How does one isolate the genomic DNA? What are the differences in DNA isolationprocedures in bacteria, plant cells and animal cells? [16]5. Explain the recent developments in DNA sequencing. [16]6. What are the different factors that one should take into consideration for obtaininga successful amplication in PCR? [16]7. What is the alternative to DNA arrays? Compare the technology and sensitivityof DNA arrays and oligo chips. [16]8. What are the limitations and advantages of gene therapy? Discuss in detail. [16]
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Code No: R05312304
Set No. 3
III B.Tech I Semester Regular Examinations, November 2007GENETIC ENGINEERING(Bio-Technology)Time: 3 hours Max Marks: 80Answer any FIVE QuestionsAll Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. What is the significant feature of the leader sequence of the amino acid biosyntheticoperons? How does this feature help in tight control of gene expression? [162. Explain the following:(a) Essential features of Leucine zipper proteins(b) Structural features of homeodomain proteins. [8
×
2]3. What properties do plasmids confer on their host cells? [16]4. How does one isolate the genomic DNA? What are the differences in DNA isolationprocedures in bacteria, plant cells and animal cells? [16]5. Explain the recent developments in DNA sequencing. [16]6. What is a primer? Discuss its role in DNA replication and add a note on itsimportance and usefulness in PCR. [16]7. Discuss the concept of gene-chip and micro-arrays in detail. [16]8. What are the possible disadvantages of producing recombinant growth hormonesfor animals? Discuss. [16]
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