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Chromatography
deals with the basics of chromatography and provides overview on different ch...
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Chromatography
Introduction to chromatography, Adsorption chromatography and paper chromatog...
What is Chromatography?
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Chromatography is a physico-chemical process thatbelongs to fractionation methods same asdistillation, crystallization or fractionated extraction.
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It is believed that the separation method in itsmodern form originated at the turn of the centuryfrom the work of Tswettto whom we attribute theterms chromatography and chromatogram
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The method was used for preparation andpurification purposes until the development ofsensitive detectors
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The detector signal, which is registered incontinuum, leads to a chromatogram that indicatesthe variation of the composition of the eluting phasewith time.
Chromatographic separations
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Sample is dissolved in a mobile phase (a gas, aliquid or a supercritical fluid)
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The mobile phase is forced through animmiscible stationary phase which is fixed inplace in a column or on a solid surface.
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The two phases are chosen so that thecomponents of the sample distributethemselves between the mobile and stationaryphase to a varying degree.
(a)Diagram showingthe separation of amixture of components A and Bby column elutionchromatography.(b)The output of thesignal detector at thevarious stages of elution shown in (a).
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What is Chromatography
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| Category: | Uncategorized. |
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| Upload Date: | 01/15/2009 |
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