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Liquid Chromatography (Chapter 28):Four types of high performance liquid chromatography (HPLC):•partition•adsorption (liquid-solid)•ion exchange•size exclusionorgel Fig 28-1:
CEM 333 page 16.1
 
Instrumentation for HPLC:•For reasonable analysis times, moderate flow rate requiredbutsmall particles (1-10
µ
m)•Solvent forced through column1000-5000 psi- more elaborateinstrument than GC•Solventsdegassed- "sparging"•High puritysolventsSinglemobile phase composition -isocratic elutionProgrammedmobile phase composition -gradientelution Fig 28-4
CEM 333 page 16.2
 
Reciprocating Pump (Fig 28-6):•Up to 10,000 psi,small internal volumes•ProducespulsationSample injection (Fig 28-7):•Similar toFIA,GC •Introduce small sample (0.1-100
µ
L)without depressurization•Microsyringe/septum system (only <1500 psi)
CEM 333 page 16.3
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