Welcome to Scribd. Sign in or start your free trial to enjoy unlimited e-books, audiobooks & documents.Find out more
Standard view
Full view
of .
Look up keyword
Like this
3Activity
0 of .
Results for:
No results containing your search query
P. 1
Investigating a novel role for eIF4A in the regulation of IL-10 by LPS and Pdcd4.

Investigating a novel role for eIF4A in the regulation of IL-10 by LPS and Pdcd4.

Ratings: (0)|Views: 30|Likes:
An essay for the 2012 Undergraduate Awards Competition by Daniel Johnston. Originally submitted for Biochemistry with Immunology at None, with lecturer Prof Luke O'Neill in the category of Medical Sciences
An essay for the 2012 Undergraduate Awards Competition by Daniel Johnston. Originally submitted for Biochemistry with Immunology at None, with lecturer Prof Luke O'Neill in the category of Medical Sciences

More info:

Published by: Undergraduate Awards on Aug 30, 2012
Copyright:Attribution Non-commercial

Availability:

Read on Scribd mobile: iPhone, iPad and Android.
See more
See less

02/06/2015

 
The University of DublinTrinity College
Investigating a novel role for eIF4A in theregulation of IL-10 by LPS and Pdcd4.
Thesis submitted as partial fulfilment of the requirements of the B.A.Moderatorship in Biochemistry with Immunology.23
rd
March 2012
Supervisors
Dr. Eva Palsson-McDermottProf. Luke O’ Neill
 
 ii
Declaration
UNIVERSITY OF DUBLINSchool of Biochemistry and ImmunologyI declare that this dissertation, in whole or in part, has not been submittedto any University as an exercise for a degree. I further declare that,except where reference is given, it is entirely my own work.The author confirms that the library may lend or copy this dissertationupon request for academic purposes.March 2012
 
 iii
Abstract
Lipopolysaccharide (LPS) induces both pro-inflammatory and anti-inflammatoryresponses upon binding to Toll-like receptor 4 (TLR4). One such anti-inflammatoryresponse is the increased production of interleukin-10 (IL-10), a cytokine thatnegatively regulates the activity of several transcription factors associated withinflammation. The expression of the tumour suppressor Programmed Cell Death 4 protein (Pdcd4) is key regulator in IL-10 production. Pdcd4 expression is regulated by multiple mechanisms in response to LPS, principally via
 Pdcd4
mRNAdegradation by MicroRNA-21 (miR-21) and phosphorylation by theAkt/mTOR/S6K1 pathway. However, the events downstream of Pdcd4 in this process are less well characterized. IL-10 mRNA contains a complex 5’ cap-structurewhich requires translation through the eIF4F complex. Pdcd4 suppresses protein production by binding eIF4A and inhibiting translation, but this interaction has not been shown in the context of LPS. Pdcd4 was seen to be degraded in response to LPSand this degradation was shown to be due to activation of mTOR. An interaction between Pdcd4 and eIF4a was also confirmed in transfected cells via co-immunoprecipitation, although the apparent stabilization of Pdcd4 conflicted with previous endogenous data. An interaction was also detected in an endogenous co-immunoprecipitation, and the pattern of Pdcd4 dissociation appeared to match that of the other endogenous experiments. The results suggest that IL-10 production inresponse to LPS may indeed be facilitated by the release of eIF4A by Pdcd4.

Activity (3)

You've already reviewed this. Edit your review.
1 thousand reads
1 hundred reads
Reja Bora liked this

You're Reading a Free Preview

Download
scribd
/*********** DO NOT ALTER ANYTHING BELOW THIS LINE ! ************/ var s_code=s.t();if(s_code)document.write(s_code)//-->