J. of Nuclear Sci. and Tech.

No. 50, pp. 29-36, 2010

1388 29-36 50

67-

43

1 3 2 1*

- 14395-836 : -1
- -2
- -3
- -3

( SPION) :
.[ 67Ga]-SPION 67-
TEM HRSEM VSM DSC TGA XRD . 1 2 Fe+2 Fe+3

SPION . FT-IR
.( RTLC %96 ) 67-
67

Ga+3 24 [ 67Ga]-SPION . 4

. .

.

67 :

Preparation and Biological Evaluation of [67Ga]-Labeled-Superparamagnetic

Iron Oxide Nanoparticles in Normal Rats
A.R. Jalilian*1, A. Panahifar2, M. Mahmoudi3, M. Akhlaghi1, A. Simchi3,4
1- Radiopharmaceutical Research and Development Lab, Nuclear Science and Technology Research Institute, AEOI, P.O. Box: 14395-836, Tehran-Iran

2- Department of Engineering and Technical, Science and Research Branch, Islamic Azad University, Tehran-Iran
3- Institute for Nanoscience and Nanotechnology, Sharif University of Technology, Tehran-Iran
4- Department of Material Science and Engineering, Sharif University of Technology, Tehran-Iran

Abstract: Gallium-67 labeled superparamagnetic iron oxide nanoparticles ([67Ga]-SPION) were

prepared and evaluated for their altered biodistribution in normal rats. SPIONs with narrow size
distribution were synthesized by co-precipitation technique using ferric and ferrous salts at molar ratio
Fe3+/Fe2+=2:1 followed by structure identification using XRD, TGA, DSC, VSM, HRSEM, TEM and
FT-IR techniques. In order to trace SPION bio-distribution, the radiolabeled iron oxide nanoparticles
were prepared using 67Ga with a high labeling efficiency (over 96%, determined by RTLC method) and
they also showed an excellent stability at room temperature for at least 4 days. The biodistribution of the
radiolabeled SPION was checked in normal male rats up to 24 hours compared with the free Ga3+ cation
biodistribution. The data strongly support the alteration of the tracer accumulation in reticuluendothelial
system while the stability of the complex is highly retained. The result is promising for determining the
position of the nanoparticles injected into a tissue when hyperthermia treatment/imaging is applied in
biomedical fields.

Keywords: Superparamagnetism, Iron Oxides, Gallium 67, Biodistribution, Labeled Compounds

*email: ajalilian@nrcam.org

88/10/15 : 87/9/12 :

 . . .

-2

-1
)(1

) (SPION

1-2

67-

30MeV Cyclone-30,IBA

) (MRI) (2 ) (3

) (FeCl3, FeCl2

.
)(5

)(6

GC1020-7500SL .

] 1 .[6

30

Bio Scan AR2000 .

].[7

2-2 SPION

0/1

) ( . FeCl2 FeCl3

) (4 [8] 64-

1 2

[9] 99m-

N2

] [10 .

pH 8

. 30

. ) -

3(

. pH

.
)

67-.
SPION

].[11

 1388 50

3-2 [67Ga]-SPION

5-2 [67Ga]-SPION

SPION

[67Ga]-SPION

. 67-

25C 4

) 5-2 67- (0/2 HCl N2

RTLC

67-

pH . HCl

6/8 . 0/22

ITLC .

.
+3

67

67

6-2 [ Ga]-SPION Ga

4-2

[67Ga]-SPION

1-4-2 Ga-SPION

67

[ Ga]-GaCl3

. TEM

. 100-50

ZEISS EM-10C HRSEM 100kV

[67Ga]-SPION

FEG LECO

) 67Ga+3 %0/9 (

10Ci

XRD Siemens D500 K

)(7

.
3

67

67

Ga+ [ Ga]-GaCl3 N2

ABB Bomem

%0/9 .

MB-100 . TGA DSC

pH 6/5.

DSC/TGA SDT Q600,

1 24

USA .

30 750C 10C/min

.
VSM . VSM

-3

(emu)10-3 8

1-3 SPION

) 66 (

1 TEM HRSEM SPION

.
TEM 25nm-15
.

2-4-2
67

5 [ Ga]-SPION

2 SPION .

/ / ) (2:4:4

) (1000cm-1-500

Rf . Ga+ SPION

. ) Fe3O4 (570cm-1

0/9 0/0 ) .(6

) -Fe2O3 (610cm-1-520 ].[12

67

 . . .

Fe-O 572cm-1
Fe3O4 .
-1

3400cm SPION

)(
.
3 TGA DSC
. SPION
.
SPION

. DSC

)(a

.
Fe3O4 Fe2O3 .

4
.

) ( .
3
. .
5nm
.
)(b

(a) -1 (b) TEM HRSEM SPION

VSM .

Transmittance

TV

)Weight (%

-1

) Wavenumber (cm

)Temperature ( C

-2 .SPION

-3 TGA DSC .SPION

 1388 50

RTLC . 6 RTLC
[67Ga]-GaCl3
[67Ga]-SPION .
counts

67-
Rf =0/9 ) (a -6
SPION
Rf =0/0 .

28

4-3 [67Ga]-SPION

-4 SPION

[67Ga]-SPION 4

. RTLC 4
%86
)magnetization (emu/g

) .(7

Counts

)Applied field (Oe

-5 .SPION
2-3 SPION

)Position (mm

)(a

SPION
.

96.23%

pH
Counts

)
( . SPION

3.27%

)Position (mm

)(b
3-3 SPION

-6 (a) RTLC [67Ga]-SPION (b) 67Ga3+

 . . .

.
)Radiochemical purity (%

] .[13


.
24
[67Ga]-SPION
. [67Ga]-SPION

)Time (h

-7 96

4 15 2/5 67Ga

) .(9

5-3

3+

67

[67Ga]-SPION

Ga

100-50 .

[67Ga]-SPION
18

) 67Ga3+ %0/9 ( 10Ci

16
14

12

[Ga]-SPION

[Ga]-GaCl3

% ID/gr

10

1 24

Tissue

-8 [67Ga]-SPION [67Ga]-GaCl3

) (ID/gr% .

1 ) [67Ga]-SPION (8

30

25

67Ga 2
[67Ga]-SPION .

[Ga]-SPION

15

[Ga]-GaCl3

10

3 5 .

22 67Ga .

[67Ga]-SPION

Tissue

67

67

-9 [ Ga]-SPION [ Ga]-GaCl3

24 .

% ID/gr
g

%3 67Ga+3 .

20

1388 50

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