BALWOIS 2010 - Ohrid, Republic of Macedonia - 25, 29 May 2010
Quality Control Methods ISO 9308 And ISO 7899 - 2 In SanitaryMicrobiology In Center For Public Health – Bitola, Macedonia
Angela Delova, Vladimir Kakurinov, Olivera Jankova, Dijana Blažekovi
, Vladimir Trajkovski Center for public healthBitola, Macedonia
Application of the standard ISO/IEC 17025 becomes imperative in ensuring system quality inlaboratories. The ISO method 9308 used bases: Lactose TTC Agar, Triptic soy Agar, DEV Tryptophan Broth, Oxydaza test and reference type E. coli ATCC 25922. The ISO 7899-2 method used bases: Slanetz and Bartley Agar, Bille Aesculine Agar and reference type Enterococcus faecalis ATCC 19433. Standard ISO 17025 facilitates cooperation among laboratories in exchanging experience and harmonize standards and procedures.
Key words: quality control method, drinking water, ISO 9308, ISO 7899- 2
Application of the standard ISO/IEC 17025 becomes imperative in ensuring system quality inlaboratories. Among the other procedures required by this standard is the procedure for evaluation ofvagueness in the microbiological laboratory testing. It defined procedures that are carried out in theintroduction of new method, equipment or changing working conditions.
Aim of this work is to display the vagueness in carrying out the measurements methods ISO 9308(detection and counting the bacteria
and coliforms with MF method and ISO 7899-2 (Detectionand counting of intestinal enterococcus with MF method in drinking water).
Materials and methods
The ISO method 9308 used bases: Lactose TTC Agar, Triptic soy Agar, DEV Tryptophan Broth,Oxydaza test and reference type
ATCC 25922. The ISO 7899-2 method used bases: Slanetzand Bartley Agar, Bille Aesculine Agar and reference type
ATCC 19433. Ascriteria for the verification method is selected the following parameters: 1. Quality control of baseswhich are used in the methods per stages with reference types; 2. Determination of method precision.In quantitative methods the precision is determined by the terms: standard deviation, relative standarddeviation. For qualitative methods are defined other performance characteristics of accuracy(sensitivity, specificity), acordation, concordation.
Ten samples of drinking water contaminated with reference type
and 10 samples of waterwithout the presence of bacteria (distilled water) were examinated. Identical results were obtainedbetween the two trials of the same sample in the same laboratory conditions as well as betweenlaboratory comparisons. When enumerating colonies of
(in 20 tests on packed samples),obtained values are within the available range ± 2 standard deviations (Shewart scheme) and therelative standard deviation 1,42% which indicates very little deviation from the average value. Thesame approach is shown for the method ISO 7899-2.