Agricultural Sci. J. 38(6) (Suppl.

): 54-57 (2007)

. . . 38(6)(): 54-57 (2550)

The Extraction of Medicinal Herbs for Inhibit Food Pathogen

1,2 1
Phongmanee, S.12 and Sanampol, G.1

Abstract
The extracted juice, steam extraction and 95% ethanol extraction of 24 medicinal herbs were studied for
their antimicrobial activities against two strains of food pathogens, Staphylococcus aureus TISTR 029 and
Salmonella typhimurium TISTR 292. It was found that steam extraction of Ka-yaeng (Limnophila aromatica Merr.)
showed highest antimicrobial activities against Staphylococcus aureus TISTR 029. It was showed 9.5 mm.
diameter of clear zone sized after 24 h. of incubation time. Extracted juice of Lead Tree (Leucaena leucocephala
(Lam.) de Wit.), 95% ethanol extraction of Pakchoi (Brassica chinensis L.) and leaf of Vegetable Humming Bird
(Sesbania grandiflora (L.) Deav.) showed highest antimicrobial activities against Salmonella typhimurium TISTR
292. Each was showed 8.0 mm. of clear zone sized after 24 h. of incubation time.
Keywords : medicinal herb, extracted herb, antimicrobial activity, food pathogen

95%
24 2 Staphylococcus
aureus TISTR 029 Salmonella typhimurium TISTR 292 (Limnophila
aromatica Merr.) S. aureus 9.5 . 24
(Leucaena leucocephala (Lam.) de Wit.) 95%
(Brassica chinensis L.) (Sesbania grandiflora (L.) Deav.) Salmonella typhimurium
8.0 . 24
:

(, 2547) (Clove)
(Cinnamon)
Salmonella typhymurium, Escherichia coli, Staphylococcus aureus, Bacillus cereus,
Bacillus subtilis mycotoxigenic Aspergillus Candida albicans
Aspergillus flavus, Aspergillus parasiticus Mycotoxigenic Aspergillus Salmonella
spp. Vibrio parahaemolyticus (Snyder, 1997)
Staphylococcus aureus Salmonella Anatum (, 2548)
Staphylococcus aureus Salmonella typhymurium

10160
Department of Food Technology, Faculty of Science, Siam University, Bangkok, 10160
2
E-mail address: suphapornp@yahoo.com
1

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38 6 .2550
55
J. 38(6) (Suppl.): 54-57 (2007)
. . 38(6)(): 54-57 (2550)

Staphylococcus aureus TISTR 029 Salmonella typhimurium TISTR 292

slant agar
1 Nutrient broth 5 . 37 oC 24
. 108 CFU/ml
24 (Metha cordifolia Opiz ex
Freser.) (Leucaena leucocephala (Lam.) de Wit.) (Telosma minor Craib) (Basella rubra L.)
(Pandanus amaryllifolius Roxb.) (Hibiscus sabdariffa L.) (Sesbania grandiflora (L.) Deav.)
(Acacia pennata (L.) Willd. subsp. Insuavis (Lace) I.C. Nielsen) (Piper
sarmentosum Roxb.) (Senna siamea (Lam.) Irwin & Barneby) (Limnophila aromatica Merr.)
(Brassica chinensis L.) (Erythrina fusea Lour.) (Tiliacora Triandra Diels) (Ocimum
basilicum L.) (Oenanthe javanica (Blume) DC.) (Anethum graveolens L.) (Centella asiatica
(L.) Urb.) (Carum carvi L.) (Erygium foetidum L.) (Melientha suavis Pierre) (Morinda
citrifolia L.) (Amaranthus lividus L.) (Azadirachta indica Valeton.)

70% 3 60 oC 3 .

70%
3

5 150 .
flask (Figure1) 4 .

95%
95% 5
150 . 150 3
4

Figure 1 Protein extraction apparatus for vapor extraction of medicinal herbs.

Staphylococcus aureus
Salmonella typhimurium Staphylococcus aureus Salmonella typhimurium
1 . Spread Nutrient Agar paper disk 5 .
spread 37oC 48 .
(clear zone) 24 . 48 .

. . . 38(6)(.):
54-57 (2550)
56 Agricultural Sci. J. 38(6) (Suppl.): 54-57
(2007)
38 6 2550

Staphylococcus aureus TISTR 029

24
Staphylococcus aureus 24 . 6 . 48 . 6.3
6.7 . (Figure 2) 95%
Staphylococcus aureus 24 8.0 8.7 9.0
8.0 . 48 9.0 9.0 8.7 7.7 .
Staphylococcus aureus 24 48 . Table 1

Figure 2 (left) Inhibition zone against Staphylococcus aureus by extracted juice of Khayaeng (right)
(Limnophila aromatica Merr.).
Table 1 Inhibition zone against Staphylococcus aureus by steam extraction of medicinal herbs.
Medicinal herbs
Mentha (Metha cordifolia Opiz ex Freser.)
Ceylon spinach (Basella albra L.)
Roselle (Hisbiscus sabdariffa L.)
Thai copper pod (Senna siamea (Lam.) Irwin & Barneby)
Khayaeng (Limnophila aromatica Merr.)
Pakchoi (Brassica chinensis L.)
Sweet basil (Ocimum basilicum L.)
Dill (Anethum graveolens L.)
Tiger herbal (Centella asiatica (L.) Urb.)

diameter of clear zone

(mm)
24 h.
48 h.
0
8.0
9.0
9.0
7.0
8.0
8.5
8.5
9.5
9.5
0
6.0
8.0
8.0
8.5
8.5
8.5
8.5

Salmonella typhimurium TISTR

292 Salmonella typhimurium
24 . 8.0 6.0 5.7 . 48 .
11.0 16.0 .
95% 24 48 . 8.0 .
95% 24 . 6.0 . 48 .
Salmonella typhimurium Table 2

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J. 38(6) (Suppl.): 54-57 (2007)
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Staphylococcus aureus
Salmonella typhimurium Salmonella typhimurium
24 . 48 .

Table 2 Inhibition zone against Salmonella typhimurium by steam extraction of medicinal herbs.
Medicinal herbs
Ceylon spinach (Basella alba Linn.)
Thai copper pod (Cassia siamea Britt.)
Khayaeng (Limnophila aromatica (Lomk.) Merr.)
Sweet basil (Ocimum basilicum L.)
Dill (Anethum graveolens L.)
Fitweed (Eryngium foetidum Linn.)

diameter of Clear zone

(mm)
24 h.
48 h.
0
7.0
0
7.5
0
8.0
0
8.7
0
8.5
0
7.0

Salmonella typhimurium
48 . 95%

95%
95%
Staphylococcus aureus Salmonella typhimurium
95% (2548)
Staphylococcus aureus

Snyder Peter O., 1997, Antimicrobial effects of spices and herb. [online], Available:
http://www.hi-tm.com / Documents/Spices.html [January/ 25/ 2007]
, 2547,
,
.
, 2548, Staphylococcus aureus , Salmonella Anatum
() ,
.