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QUALITY CONTROL
The media, strips, and reagents are systematically quality controlled at various stages of their manufacture. For those who wish to
perform their own quality control tests with the strip, it is recommended that the following stock cultures be used, to obtain the results
below : Table 1
VP HIP ESC PYRA _GAL _GUR _ GAL PAL LAP ADH RIB ARA MAN SOR LAC TRE INU RAF AMD GLYG
1. – – – – – + – + + + + – – + + – – – + +
2. + V + + + V + – V + + + + – + + + + + –
3. + + + V V + – V + + + – + + + + + + – –
4. V V + V V – – – – – – – + – – + – + + –
1. Streptococcus equi ssp zooepidemicus ATCC 700400 3. Streptococcus uberis ATCC 700407
2. Enterococcus gallinarum ATCC 700425 4. Aerococcus viridans ATCC 700406
ATCC : American Type Culture Collection, 10801 University Boulevard, Manassas, VA 20110-2209, USA.
• Inoculum adjusted to between 4.5 and 5.5 McF.
• Profiles obtained after : - 4 hours of incubation for tests VP to LAP
- 24 hours of incubation for tests ____
ADH to _____
GLYG
• Strains cultured on Columbia sheep blood agar
Table 2
TESTS SUBSTRATES REACTIONS/ENZYMES RESULTS
NEGATIVE POSITIVE
_GAL 6-Bromo-2-naphthyl
_-D-galactopyranoside _-galactosidase Colorless Violet
PAL 2-naphthyl phosphate Alkaline phosphatase Colorless or very pale violet Violet
ADH
____ Arginine Arginine dihydrolase Yellow Red
RIB
___ Ribose Acidification Red Orange/Red Orange/Yellow Yellow
ARA
____ L-Arabinose Acidification Red Orange/Red Orange/Yellow Yellow
MAN
____ Mannitol Acidification Red Orange/Red Orange/Yellow Yellow
SOR
____ Sorbitol Acidification Red Orange/Red Orange/Yellow Yellow
LAC
____ Lactose Acidification Red Orange/Red Orange/Yellow Yellow
TRE
____ Trehalose Acidification Red Orange/Red Orange/Yellow Yellow
INU
___ Inulin Acidification Red Orange/Red Orange/Yellow Yellow
RAF
____ Raffinose Acidification Red Orange/Red Orange/Yellow Yellow
AMD
____ Starch (2) Acidification Red Orange/Red Orange/Yellow Yellow
GLYG
_____ Glycogen Acidification Red or Orange Bright yellow
(1) During a second reading after 24 hours of incubation, a deposit may be noticed in the tubes where the ZYME A and ZYME B reagents have been added.
This phenomenon is normal and should not be taken into consideration.
(2) The acidification of starch is frequently weaker than that of other sugars.
(3) A pale pink color obtained after 10 minutes should be considered negative.
0
api 20 Strep 07625 B - 08/97
RECOMMENDED METHODOLOGY
- Cocci
- Gram +
- Catalase −
Blood agar
24:00 02 37°C
> 4 McF
Suspension Medium 2 ml
VP ADH
ADH
GP Medium
RIB GLYG
RIB GLYG
4:00 37°C
+ - + - + -
IDENTIFICATION TABLE
Table 3
% of positive reactions after 4/24 hrs. at 35-37°C
API 20 STREP V6.0 VP HIP ESC PYRA AGAL BGUR BGAL PAL LAP ADH RIB ARA MAN SOR LAC TRE INU RAF AMD GLYG HEM
Aerococcus viridans 1 13 50 96 54 33 16 37 1 5 1 83 33 85 70 83 99 33 41 70 33 1
Aerococcus viridans 2 15 70 50 76 10 20 25 1 5 5 25 1 35 2 70 89 1 5 24 1 5
Aerococcus viridans 3 22 88 99 40 85 48 14 14 1 1 8 2 82 5 91 99 37 99 14 1 1
Enterococcus faecium 94 43 99 95 42 1 90 3 97 93 85 70 78 18 84 98 26 9 73 3 1
Gardnerella vaginalis 0 95 0 0 0 1 53 0 99 0 46 6 1 0 1 0 0 0 73 53 0
Gemella haemolysans 25 0 0 70 0 0 1 84 40 1 1 0 20 10 5 2 0 0 10 5 1
Gemella morbillorum 3 0 0 35 0 0 10 35 86 4 5 0 1 0 1 11 3 1 16 5 0
Leuconostoc spp 91 1 60 5 55 0 65 2 70 10 37 35 29 4 35 65 0 42 11 0 0
Listeria spp 97 79 98 0 0 0 0 0 85 0 6 0 0 0 49 92 1 1 72 0 26
Abiotrophia adiacens 0 0 10 80 0 25 0 0 99 0 0 0 0 0 0 0 0 0 0 0 0
Streptococcus acidominimus 1 95 4 13 1 66 30 60 96 18 17 0 42 10 70 65 0 0 10 0 0
Streptococcus equi ssp equi 1 0 1 0 0 100 0 100 100 100 0 0 0 0 0 1 0 0 100 100 100
Streptococcus mitis 1 1 0 3 1 21 0 25 35 99 19 14 1 0 1 94 7 3 26 67 5 0
1. APPELBAUM P.C., CHAURUSHIYA P.S., JACOBS M.R., 6. HUMAN R.P. and TILLOTSON G.S.
DUFFETT A. Identification of Gardnerella vaginalis with the API 20 Strep
Evaluation of the Rapid Strep System for Species System.
Identification of Streptococci. (1985) J. Clin. Microbiol., 21, 985-986.
(1984) J. Clin. Microbiol., 19, 588-591. 7. KLOOSTERMAN R.E., CULLEN K.D.
2. BALL L.C., COLMAN G. Comparison of Two Commercial Systems for the Rapid
A Comparison of Conventional Methods and API Galleries Identification of Streptococci.
for the Identification of Streptococci. (1984) ASM ST. LOUIS C198.
(1982) International Meeting on Streptococci and 8. MacGOWAN A.P., MARSHALL R.J., REEVES D.S.
Streptococcal Diseases, LUND SWEDEN, 41-42. Evaluation of API 20 STREP System for Identifying Listeria
3. BANNISTER M.F., BENSON C.E. and SWEENEY C.R. Species.
Rapid Species Identification of Group C Streptococci (1989) J. Clin. Path., 42, 548-550.
Isolated from Horses. 9. RUOFF K.L., KUNZ L.J.
(1985) J. Clin. Microbiol., 21, 524-526. Use of the Rapid STREP System for Identification of
4. COLMAN G., BALL L.C. Viridans Streptococcal Species.
Identification of Streptococci in a Medical Laboratory. (1983) J. Clin. Microbiol., 18, 1138-1140.
(1984) J. Appl. Bact., 57, 1-14. 10. TILLOTSON G.S.
5. FACKLAM R.R., RHODEN D.L., SMITH P.B. An Evaluation of the API 20 Strep System.
Evaluation of the Rapid Strep System for the Identification (1982) J. Clin. Path., 468-471.
of Clinical Isolates of Streptococcus Species.
(1984) J. Clin. Microbiol., 20, 894-898.
Fabricant / Manufacturer bioMérieux SA