669; doi: 10.1038/oby.2005.74
Regulation of Adiponectin Expression in HumanAdipocytes: Effects of Adiposity, Glucocorticoids, andTumor Necrosis Factor
Division of Endocrinology and Metabolism, Department of Medicine, IndianaUniversity School of Medicine; Indianapolis, Indiana2.
Department of Surgery, Indiana University School of Medicine; Indianapolis, Indiana3.
St. Vincent Bariatric Services, Carmel, Indiana4.
Department of Clinical Biochemistry, Addenbrook's Hospital, Cambridge, UnitedKingdomCorrespondence: Robert V. Considine, Indiana University School of Medicine, 541 NorthClinical Drive, Clinical Building 455, Indianapolis, IN 46202-5111. E-mail: firstname.lastname@example.org
The costs of publication of this article were defrayed, in part, by the payment of page charges.This article must, therefore, be hereby marked "advertisement" in accordance with 18 U.S.C.Section 1734 solely to indicate this fact.Received 9 July 2004; Accepted 14 January 2005.Top of page
Adiponectin mRNA expression in isolated subcutaneous and omental adipocytes wasexamined across a wide range of adiposity to determine whether adiponectin synthesis isimpaired in these adipose tissue depots in obese humans. Tumor necrosis factor (TNF) anddexamethasone were tested for inhibitory effects on adiponectin release from human adipocytesin vitro.
Research Methods and Procedures:
Adipocytes were isolated by collagenase digestion of abdominal adipose tissue obtained from subjects undergoing surgical procedures or outpatientneedle biopsy. Adiponectin and leptin mRNA were quantitated by real-time reverse