ABSTRAK Caesaria Christ Haryadi, G0009041, 2012, Pengaruh Ekstrak Buah Naga Putih ( Hylocereus undatus ) sebagai Nefroprotektor

terhadap Kerusakan Histologis Sel Ginjal Mencit yang Diinduksi Parasetamol, Fakultas Kedokteran Universitas Sebelas Maret Surakarta. Tujuan Penelitian: Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak buah naga putih terhadap kerusakan sel ginjal mencit yang diinduksi parasetamol dan untuk membuktikan bahwa peningkatan dosis ekstrak buah naga putih dapat meningkatkan efek proteksi terhadap kerusakan sel ginjal mencit yang diinduksi parasetamol. Metode Penelitian: Penelitian ini bersifat eksperimental laboratorik dengan post test only controlled group design. Sampel berupa mencit jantan, galur Swiss webster berumur 2-3 bulan dengan berat badan 20 g. Sampel diambil dengan teknik Randomized Controlled Trial (RCT). Sampel sebanyak 28 mencit dibagi dalam 4 kelompok, masing-masing kelompok terdiri dari 7 mencit. Mencit pada kelompok kontrol (KK) dan kelompok perlakuan 1 (KP1) diberi aquades, sedangkan kelompok perlakuan 2 (KP2) dan kelompok perlakuan 3 (KP3) diberi ekstrak buah naga putih. Parasetamol diberikan pada kelompok KP1, KP2, dan KP3 pada hari ke-12, 13, dan 14. Hari ke-15, mencit dikorbankan dengan cara neck dislocation kemudian ginjal mencit dibuat preparat dengan metode blok parafin dan pengecatan Hematoksilin Eosin (HE). Gambaran histologis ginjal diamati dan dinilai berdasarkan jumlah kerusakan histologis yang berupa penjumlahan inti piknosis, karioreksis, dan kariolisis. Data dianalisis dengan menggunakan uji One-Way ANOVA ( = 0,05) dan dilanjutkan dengan uji Post Hoc Multiple Comparisons (LSD) ( = 0,05). Hasil Penelitian: Hasil uji One-Way ANOVA menunjukkan adanya perbedaan yang bermakna antara keempat kelompok. Hasil uji LSD menunjukkan adanya perbedaan yang bermakna antara KK-KP1, KK-KP2, KK-KP3, KP1-KP2, KP1-KP3, dan KP2-KP3. Simpulan Penelitian: Ekstrak buah naga putih dapat mengurangi kerusakan sel ginjal mencit yang diinduksi parasetamol dan peningkatan dosis ekstrak buah naga putih dapat meningkatkan efek proteksi terhadap kerusakan sel ginjal mencit.

Kata kunci: ekstrak buah naga putih, parasetamol, kerusakan sel ginjal mencit

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ABSTRACT Margareta Grace Hartati, G0008124, 2011, The Effect of Red Paprika (Capsicum annuum var. grossum) Juice as Nefroprotector to Renal Cell Damaging of Mice that be Induced by Paracetamol, Faculty of Medicine Sebelas Maret University Surakarta. Objective: Red Paprika has antioxidant as a protection of free radicals and reducing NAPQI which produced by paracetamol metabolism. The objective are to know the effect of red paprika to the renal cell damaging of mice which is induced by paracetamol and the increase of red paprika dose can be also increase protection effect to the renal cell damaging of mice which is induced by paracetamol. Methods: This was laboratory experimental research with post test only controlled group design. Samples in this research were twenty eight male mices (Mus musculus), Swiss webster type, 2-3 months old age and 20 g of each weight. Samples divided into 4 groups, each group has seven mice. Mice for control group (KK) will not be given paracetamol and red paprika, it was only given aquades x ml/20 g weight of mice for 14 days in a row. The first treatment group (KP1) will be given paracetamol with dose 0,05 ml/20 g weight of mice on the day 12, 13 and 14. The second treatment group (KP2) will be given red paprika juice dose I x ml/20 g weight of mice for 14 days in a row and also paracetamol dose 0,05 ml/ 20 g weight of mice on day 12, 13 and 14. The third treatment group (KP3) will be given red paprika juice dose II 2x ml/20 g weight of mice for 14 days in a row and also paracetamol dose 0,05 ml/ 20 g weight of mice on day 12, 13 and 14. Finally on day 15th, mice are sacrificed with neck dislocation. After that, we made preparation from the renal that stained by Hematoxillin Eosin (HE). Renal histological is observed and scored base on quantifying of renal histological damaging on pyknosis, karyorrhexis, and karyolysis. Data were analized by One-Way ANOVA test (= 0,05), and continued by Post Hoc Multiple Comparisons test (LSD) (= 0,05). Results: Result of One-Way ANOVA showed that there was a significant of degree between 4 groups. Result of LSD method showed that there were a significant of degree between KK-KP1, KK-KP2, KK-KP3, KP1-KP2, KP1-KP3 and KP2-KP3 groups. Conclusion: The feeding of red paprika juice was able to decrease the renal cell damaging of mice (Mus musculus) and the increase of red paprika dose was followed by the increase of protection effect to the renal cell damaging of mice which was induced by paracetamol. Keywords : red paprika juice, paracetamol, renal cell damaging