Welcome to Scribd, the world's digital library. Read, publish, and share books and documents. See more
Download
Standard view
Full view
of .
Save to My Library
Look up keyword or section
Like this
1Activity

Table Of Contents

0 of .
Results for:
No results containing your search query
P. 1
Bio Molecules

Bio Molecules

Ratings: (0)|Views: 5,225|Likes:
Published by VRampriya

More info:

Categories:Types, Research, Science
Published by: VRampriya on Oct 11, 2012
Copyright:Attribution Non-commercial

Availability:

Read on Scribd mobile: iPhone, iPad and Android.
download as PDF, TXT or read online from Scribd
See more
See less

12/19/2013

pdf

text

original

 
HPLCAnalysisofBiomoleculesTechnicalGuide
 Analyze DetectMeasure Control
c h r o m a t o g r a p h y
Successful Separations of Peptides, Proteinsand Other Biomolecules
 
L   C  a l    y  s i    s  o f   i    o m o l    e  c  u l    e  s 
2
CommitmenttoCustomer Support
Thermo also offers expert, reliable, and responsivetechnical support to help you get the best chromato-graphic data possible. Our experienced technicalsupport chemists are available to help with anychromatography, mass spectroscopy, or applicationquestion quickly and accurately.
 AboutThermoElectron Corporation
In science and in business, we make progress onlywhen we dare to ask questions. Whether it
s gettinga breakthrough drug to market quickly, improvingworkplace safety, or manufacturing a product morepro
tably, Thermo and our customers around theworld are united in the search for answers. As aglobal provider of analytical instrumentation, Thermooffers an integrated resource for the best-in-classtechnology, products, and services necessary toestablish and maintain a competitive advantage.Thermo headquarters are located in Waltham,MA, USA with manufacturing locations
around theworld. Worldwide sales and service of
ces
and twodedicated Thermo Institutes in the USA and Europedeliver the ultimate in training and support for all ourinstrumentation and applications.
Dedication toLife Science
It has been said that we exist today in the Age ofBiotechnology. Major advances in the realms ofproteomics, biochemistry, and molecular biologywill enable new therapeutic treatments and increaseour understanding of fundamental life processes.As a manufacturer of HPLC and LC/MS systems,software, HPLC modular components, HPLC particles,columns, and other consumables, Thermo Electron is
in a unique position to provide life science researchers
the tools, technologies, and integrated solutionsneeded to tackle the formidable analyticalchallenges they face daily.
 
3
HPLCModesfor Biomolecule Separations
Introduction5
Reversed Phase Chromatography 
Reversed Phase HPLC of Biomolecules6Mechanism of RP-HPLC Separation6Particle Size6Pore Size7Column Length8Flow Rate8Bonded Phase Functionality and Alkyl Chain Length9Porous Graphitic Carbon (PGC)10Guidelines for Choosing an RP-HPLC Column12Mobile Phases for RP-HPLC12Effect of pH on RP-HPLC Analyses13Effect of Ionic Modi
er on RP-HPLC Analyses13Effect of Organic Modi
er Type and Concentration on RP-HPLC Retention14Effect of Temperature on RP-HPLC Retention15Gradient vs. Isocratic Elution15RP-LC/MS of Biomolecules16Column Selection for RP-LC/MS: Column ID16Maintaining Constant Linear Velocity with Changing Column ID17Electrospray Ionization (ESI)17Column Packing Materials for RP-LC/MS18Column Stability19Mobile Phase for RP-LC/MS20Ionic Additives for RP-LC/MS20Organic/Aqueous Solvent Ratio for RP-LC/MS21Effect of Connecting Tubing on LC/MS Ef
ciency21
Ion Exchange Chromatography 
Basic Principles of Ion Exchange Chromatography23Particles for Ion Exchange24Particle Size, Pore Size, and Column Length24Flow Rate24Temperature24Ion Exchange Bonded Phases25Measuring Ion Exchange Capacity25Mobile Phases for Ion Exchange HPLC26Mobile Phase pH26Ionic Strength (Salt Concentration)26Controlling Retention with Salt and pH Gradients27Ion Exchange for LC/MS of Biomolecules27
Table ofContents

You're Reading a Free Preview

Download
/*********** DO NOT ALTER ANYTHING BELOW THIS LINE ! ************/ var s_code=s.t();if(s_code)document.write(s_code)//-->