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Lo ChiMing

Lo ChiMing

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Published by Luong Cao THang

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Published by: Luong Cao THang on Oct 24, 2012
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01/18/2014

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CELLULASE PRODUCTION BY
TRICHODERMA REESEI 
RUT C30A DissertationPresented toThe Graduate Faculty of The University of AkronIn Partial Fulfillmentof the Requirements for the DegreeDoctor of PhilosophyChi-Ming LoAugust, 2008
 
CELLULASE PRODUCTION BY
TRICHODERMA REESEI 
RUT C30Chi-Ming LoDissertationApproved: Accepted:Advisor Department ChairDr. Lu-Kwang Ju Dr. Lu-Kwang JuCommittee Member Dean of the CollegeDr. Amy Milsted Dr. George K. HaritosCommittee member Dean of the Graduate SchoolDr. Fred Choy Dr. George R. NewkomeCommittee Member DateDr. Stephanie LopinaCommittee MemberDr. Steven ChuangCommittee MemberDr. Weiping Zhengii
 
 iiiABSTRACTAs the price of oil continues to increase and the concern over global warmingheightens, finding alternative renewable sources of energy becomes more and moreimperative. Considering the abundance of lignocellulosic biomass, the potentialsignificance of its conversion to fuel such as ethanol has long been recognized.Ethanol production from lignocellulosic biomass requires the breakdown of the solidmaterial to simple sugars that can be consumed by microorganisms. The breakdownof lignocelluloses includes the important step of hydrolyzing cellulose, which are
β
-1,4 linked polymers of glucose, by a group of enzymes collectively termed cellulase.The cost of cellulase production profoundly influences the economics of the entireethanol production process.
Trichoderma reesei
Rut C-30 is the most commonly usedfungal strain for industrial cellulase production. With an overall goal of decreasing thecellulase production cost, this thesis work was focused on two topics: (1)investigation of the performance of different cellulase-inducing substrates in
T. reesei
 Rut C-30 fermentation and (2) development of a more advanced model to describe theculture behaviors of 
T. reesei
Rut C-30 grown on lactose-based media. The

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