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Fish Histology Manual v4 S. Turkmen

Fish Histology Manual v4 S. Turkmen

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Published by Serhat Turkmen

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Published by: Serhat Turkmen on Oct 29, 2012
Copyright:Attribution Non-commercial

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08/30/2013

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Fish Histology and Histopathology
Contributing authors:Sonia Mumford; Jerry Heidel; Charlie Smith; John Morrison; Beth MacConnell; Vicki Blazer
Table of Contents
Sonia Mumford
John Morrison
John Morrison; Sonia Mumford
Charlie Smith
Jerry Heidel; Charlie Smith
Beth MacConnell; Vicki Blazer; Sonia Mumford; Charlie Smith; John Morrison
Vicki Blazer; Charlie Smith
 
Chapter 1 – Tissue Processing
Fish Histology and Histopathology 
July 2007
 
1 – 1
 
USFWS-NCTC 
 
Processing Tissues For Histology
Turning the Gross Tissues into Beautiful Sections
Sample collection
•Samples should be collected on freshly dead fish, not frozen or “Extremely Dead” •Sample volume should not exceed 1/10
th
of the volume of fixative•Samples should be placed in an appropriate fixative.•Fry can be fixed whole
 –
preferably in Davidson’s•Fingerlings and smaller
 –
Gill opercula cut off 
 –
Cut along midline
 –
 Viscera pulled out•Larger Fish
 –
Take samples in field
 –
Samples should be no thicker than 3mm
Fixation
•Singularly the most important step in producing good histologic slides (Sheehan)
Fixation with Formalin
•Most widely used fixative
 –
Must be buffered (pH is as low as 3 without buffering), prevents formalinpigment
 –
Penetrates 2 mm in 4 hrs, 10 mm in 24 hrs
 –
Tissue Thickness- <3 mm thick for good fixation
 –
Does not produce “overfixation” 
Tissues do not harden unpredictably
 
Chapter 1 – Tissue Processing
Fish Histology and Histopathology 
July 2007
 
1 – 2
 
USFWS-NCTC 
 
Fixation with Davidson’s
 
Contains acetic acid
 –
 
Can cause tissue swelling –
 
Results in some decalcification –
 
Should not be used if red blood cell morphology is needed (destroyscells)
 
Tissues should be transferred to 70% EtOH after 12-24 hrs to:
 –
 
Prevent tissues from over-hardening –
 
Easier to store/transport
Cutting in of Tissue - Placing tissues in cassettes
Handle Tissues Gently
Do not over-stuff Cassettes

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