Welcome to Scribd. Sign in or start your free trial to enjoy unlimited e-books, audiobooks & documents.Find out more
Standard view
Full view
of .
Look up keyword or section
Like this

Table Of Contents

0 of .
Results for:
No results containing your search query
P. 1
Fish Histology Manual v4 S. Turkmen

Fish Histology Manual v4 S. Turkmen

|Views: 265|Likes:
Published by Serhat Turkmen

More info:

Published by: Serhat Turkmen on Oct 29, 2012
Copyright:Attribution Non-commercial


Read on Scribd mobile: iPhone, iPad and Android.
download as PDF, TXT or read online from Scribd
See more
See less





Fish Histology and Histopathology
Contributing authors:Sonia Mumford; Jerry Heidel; Charlie Smith; John Morrison; Beth MacConnell; Vicki Blazer
Table of Contents
Sonia Mumford
John Morrison
John Morrison; Sonia Mumford
Charlie Smith
Jerry Heidel; Charlie Smith
Beth MacConnell; Vicki Blazer; Sonia Mumford; Charlie Smith; John Morrison
Vicki Blazer; Charlie Smith
Chapter 1 – Tissue Processing
Fish Histology and Histopathology 
July 2007
1 – 1
Processing Tissues For Histology
Turning the Gross Tissues into Beautiful Sections
Sample collection
•Samples should be collected on freshly dead fish, not frozen or “Extremely Dead” •Sample volume should not exceed 1/10
of the volume of fixative•Samples should be placed in an appropriate fixative.•Fry can be fixed whole
preferably in Davidson’s•Fingerlings and smaller
Gill opercula cut off 
Cut along midline
 Viscera pulled out•Larger Fish
Take samples in field
Samples should be no thicker than 3mm
•Singularly the most important step in producing good histologic slides (Sheehan)
Fixation with Formalin
•Most widely used fixative
Must be buffered (pH is as low as 3 without buffering), prevents formalinpigment
Penetrates 2 mm in 4 hrs, 10 mm in 24 hrs
Tissue Thickness- <3 mm thick for good fixation
Does not produce “overfixation” 
Tissues do not harden unpredictably
Chapter 1 – Tissue Processing
Fish Histology and Histopathology 
July 2007
1 – 2
Fixation with Davidson’s
Contains acetic acid
Can cause tissue swelling –
Results in some decalcification –
Should not be used if red blood cell morphology is needed (destroyscells)
Tissues should be transferred to 70% EtOH after 12-24 hrs to:
Prevent tissues from over-hardening –
Easier to store/transport
Cutting in of Tissue - Placing tissues in cassettes
Handle Tissues Gently
Do not over-stuff Cassettes

Activity (4)

You've already reviewed this. Edit your review.
1 thousand reads
1 hundred reads
ІМаринич liked this
Lindsey Waxy liked this

You're Reading a Free Preview

/*********** DO NOT ALTER ANYTHING BELOW THIS LINE ! ************/ var s_code=s.t();if(s_code)document.write(s_code)//-->