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m
s
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K S
1. Effect of Carbon Source
The concentrate of glucose or salt solution can cause
osmoticlysis in the cell of micro-organism, water will
pass in through the cell membrane and out from the
cell and it will death.
High substrate concentration that are significantly
above stoichiometric requirements are inhibitory to
cellular function.
Inhibitory levels of substrates vary depending on the
type of cells and substrate.
Glucose may be inhibitory at concentrations above
200g/l. probably due to a reduction in water activity .
2. Effect of Nitrogen Source
Nitrogen (N) is second to carbon in terms of
quantity and economic importance.
It consist approximately 10 % of the dry
weight.
It serves as the building block for the
syntheses of proteins and other cellular
macro-molecules.
Ammonia gas and solution is popular in the
industry assure of nitrogen because it is
inexpensive and easy to use.
Other sources of nitrogen in fermentation
media include corn steep liquor, fish meal,
yeast extract, and protein hydrolysate .
UNTUK PERTUMBUHAN LACTOBACILLUS
PEPTON 10 g
MEAT EXTRACT 10 g
YEAST EXTRACT 5 g
GLUCOSE 2O g
TWEEN 80 1 ml
DIKALIUM HIDROGEN PHOSPHAT 2 g
SODIUM ACETAT 2 g
DIAMMONIUM CTRAT 5 g
MAGNISIUM SULPHAT 0.2 g
MANGAN SULPHAT 0.05 g
DISTILLED WATER 1000 ml
(UTK MEDIA AGAR DITAMBAH 15 g agar)
3. Effect of Dissolved Oxygen
Some micro-organism need oxygen for growth.
Micro-organism which must have oxygen for growth
are called strict or obligate aerobes.
Strict or obligated anaerobes grow only when oxygen
is absent ; these organism occur in river mud and in
the rumen.
Microorganisme which normally grow in the
presence of oxygen but which can still grow under
anaerobic condition (absence of oxygen) are called
facultative anaerobes.
Similarly, those which normally grow anaerobically
but which can grow in the present of oxygen are
called facultative aerobes.
3. Effect of Dissolved Oxygen
For all organism, including obligate aerobes,
oxygen may be toxic at any concentration.
The mechanism of oxygen toxicity is
through the formation of single oxygen,
superoxida radicals O
2-
, peroxida O
2
2
or
hydroxy free radical OH
-
which are
destructive to many cell component.
STERILISASI
Cara Alat/Kondisi/Bahan Bahan yang
diseteril
1 Pemanasan Basah
(uap)
Autoklaf/ 15-40 menit
suhu `121
o
C
Larutan Pekat,
alat kaca, dan
plastik
2 Pemanasan Kering
a. Pemanasan Lngsung Api bunsen Kawat ose
b. Pemanasan Tidak
Langsung
Oven/60-90 menit
suhu 160
o
C
Alat-alat gelas,
bahan padat
3 Pasturisasi
(pemanasan)
30 menit suhu 62
o
C
Makanam damn
Minuman
STERILISASI
Cara Alat/Kondisi/Bahan Bahan yang
diseteril
4
Tindalisasi
(pemanasan)
30 menit suhu 100
o
C berkali-
kali
Media fermentasi
5
Kimia Formaldehid, alcohol,
hidrogen peroksida,
Lysol,merkuri chloride,
ethylene oksida, halogen
Alat gelas,
ruangan,
bahan yang
tidak tahan
panas
6
Radiasi Lampu UV Plastik, Petri dish
dll.
7
Filtrasi Kapas/ filter Cairan, Udara
/gas
Kurva Pertumbuhan Mikroorganisme
1. Lag phase, 2. Exponential phase, 3. Logarithmic phase, 4. Stationer phase and 5. Death phase