International Transaction Journal of Engineering,Management, & Applied Sciences & Technologies
Morphological Change Due to Effects of Acute Gamma Ray onWishbone Flower (
Department of Biotechnology, Faculty of Science and Technology, Thammasat University,THAILAND
A R T I C L E I N F O
A B S T RA C T
:Received 17 June 2011Received in revised form01 August 2011Accepted 03 August 2011Available online03 August 2011
:Wishbone Flower,Gamma Rays,Acute Irradiation,Morphology,Tissue Culture
Young shoot tips were used as explants and cultured onMS medium supplemented with varying concentrations of (0.1,0.5, 1.0 mg/l) BA and (0.1, 0.5, 1.0 mg/l) NAA. Calli and newshoots were grown on MS medium supplemented withcombination of 0.5 mg/l BA and 0.5 mg/l NAA. New shootsaveraging 0.5–0.8 cm were irradiated with varying doses of gamma rays (5, 10, 15, 20 grays). Gamma irradiation hadvarious effects on growth of
. Higher dosage of gamma irradiation reduced plant height, number of roots, number of leaves, leaf length, leaf width, petiole length and number of guard cells at abaxial and adaxial epidermis surface. Plantmorphology and flower development was also modified.
2011 International Transaction Journal of Engineering, Management, &Applied Sciences & Technologies. Some Rights Reserved.
Wishbone flower or
is a member of the Scrophulariaceae family. It isgenerally a perennial plant which is normally grown as an annual shrub. With an approximateheight of 12 inches, it is preferably planted along with many other similar species to ensure awidespread flowering bed. The plant looks like a nice little green shrub. The mature plant isdensely branched and decorated with shiny green leaves and delicate cup-shaped flowers. It isgrown as a pot plant or used for decorating and landscaping. Demand for the plant has