Welcome to Scribd, the world's digital library. Read, publish, and share books and documents. See more
Download
Standard view
Full view
of .
Look up keyword
Like this
3Activity
0 of .
Results for:
No results containing your search query
P. 1
Diagnosis of Syphilis

Diagnosis of Syphilis

Ratings:

5.0

(1)
|Views: 169 |Likes:
Published by Examville.com
For more FREE Microbiology Study Material visit www.examville.com
For more FREE Microbiology Study Material visit www.examville.com

More info:

Categories:Types, School Work
Published by: Examville.com on Feb 02, 2009
Copyright:Attribution Non-commercial

Availability:

Read on Scribd mobile: iPhone, iPad and Android.
download as PDF or read online from Scribd
See more
See less

05/09/2013

 
 APPLIED MICROBIOLOGY
 
LABORATORY DIAGNOSIS OF SYPHILIS
Syphilis is caused by a treponeme, Treponema pallidum. The disease runs in stages. The diagnosismay be required in its primary stage, secondary stage, tertiary stage or late syphilis as well as treatedand congenital syphilis.Laboratory diagnosis is indicated for:a. Diagnosing a suspected case of syphilisb. Assessment of efficacy of treatmentc. Screening of blood and preventing spreadThe diagnosis of syphilis can be established by:a. Demonstration of treponema by microscopy in congenital syphilis and primary and secondarystagesb. Demonstration of reaginic and treponemal antibodies.
Direct Examination
Detection of treponemes from the lesion is the key aid to diagnosis. Dark-field microscopy is recom-mended. Samples should be collected under aseptic conditions before antibiotics are started.Organisms of T. pallidum are actively motile filamentous helicals with evenly spaced coils.
Immunofluorescence
Tissue fluid or exudate is spread on a glass slide, air dried and sent to the laboratory. It is fixed andstained with fluorescent labelled anti-treponeme serum and examined by means ofimmunofluorescent microscopy. This gives a higher positivity rate than the direct microscopy.
Serological Tests
These tests form the mainstay of laboratory diagnosis. It is useful to carry out at least two differenttests on each specimen. Two types of antigens are available are:
a. Cardiolipin:
not derived from spirochaetes and used in VDRL (Venereal Diseases ResearchLaboratory) tests which are non-treponemal test.
b. Specific antigens derived from T.
pallidum which are used in TPHA (Treponema pallidumhaemagglutination test) and fluorescent treponema antibody absorbed (FTA-Abs) tests.In routine three serological tests (VDRL, TPHA, FTA-Abs) are used.
VDRL Test
Venereal Diseases Research Laboratory (VDRL) test is based on the fact that the particles of lipidantigens remain dispersed with normal serum but form visible clumps when combine with reagin.VDRL is a type of flocculation test which can be performed on a slide as well as in a tube. In slide test,0.5 ml of serum of the patient is taken in special slides with depressions (cavity slides) or slidesprepared with paraffin rings.
 
 One drop of freshly prepared antigen is added with a syringe delivering 60 drops from one ml. Theslide is shaken in a VDRL shaker for 4 minutes at 180 rotations. It is then examined under the micros-cope with low power objective. Formation of clumps indicates positive reaction designated asreactive while uniform distribution of crystals in the drop indicates that the serum is non-reactive. Aweakly positive reaction may also occur (weakly reactive).The reactive sera can be diluted and results quantified. VDRL test can also be done in tubes as tubeflocculation test.
Advantages of VDRL test:
a. Simple and rapid testb. Reasonably sensitive and specificc. Requires small quantity of serumd. Can be easily quantifiede. Results are reproduciblef. Reagents are cheap, easily available and have long shelf lifeg. Quality control of tests can be performed.
Biological False Positive Results
In spite of simplicity of test and numerous advantages there are various biological false positive(BFP) results. These can be classified as acute (when they disappear in six months time) or chronicwhen they are positive for more than six months), include the following:Systemic lupus erythematosus, Malaria, Lepromatous leprosy, Infectious mononucleosis, Tropicaleosinophilia, Relapsing fever, Hepatitis, Tissue regeneration, Collagen disorders, Severe trauma,Coronary artery disease, Repeated blood loss, Menstruation, Immunization, Pregnancy, Haemolyticanaemia and Heroin addiction.
TPHA Test
Treponema pallidum haemagglutination (TPHA) test is performed by using extract of T. pallidum.Red blood cells are treated to adsorb treponemes on their surface. When mixed with serumcontaining antitreponemal anti bodies, the cells become clumped. This test is similar to FTA-ABS testin specificity and sensitivity, but become positive somewhat later in the infection.Micro haemagglutination -T. pallidum (MHA-TP) is automated version of this test and so also ishaemagglutination treponemal test for syphilis (HATTS). However, these two tests lack sensitivity inprimary syphilis.
FTA-ABS Test
Fluorescent antibody test uses an indirect immunofluorescence test in which Nichol's strain of T.pallidum is employed as antigen. The patient's serum is diluted 1:5 and reacted with the antigenwhich has been smeared on the slide. The combination is covered with antihuman immunoglobulinfluorescent conjugate. After proper washing, the smear is examined under fluorescent microscope.In a positive test fluorescent treponemes are observed. When patient serum is diluted to 1:200 insteadof 1:5 (as is done now a days), the test is called as FTA200. Patient's serum is adsorbed with an extractof Reiter's treponemes to avoid false positive results and then the test is performed (FTA-ABS test).This is extremely sensitive and specific test.
 
 Interpretation of Serological Tests for SyphilisStage of disease VDRL TPHA FT A-ABS
Primary syphilis +/- +/- +Secondary syphilis + + +Tertiar shilis + + +Late syphilis + + +Conenital shilis + + +Treated syphilis - + +
The nontreponemal tests are mainly used for:
a. screening proceduresb. to detect reinfectionc. to evaluate response to therapyd. to diagnose neurosyphilis.
The treponemal tests are mainly used for:
a. confirmation of diagnosis of syphilisb. detection of latent syphilis andc. diagnosis in patients with negative non treponemal tests.

Activity (3)

You've already reviewed this. Edit your review.
1 hundred reads
fathan liked this
Aji Setia Utama liked this

You're Reading a Free Preview

Download
scribd
/*********** DO NOT ALTER ANYTHING BELOW THIS LINE ! ************/ var s_code=s.t();if(s_code)document.write(s_code)//-->