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kinetics

kinetics

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Published by Joanna Hoang
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Published by: Joanna Hoang on Dec 07, 2012
Copyright:Attribution Non-commercial

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06/09/2014

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Microbial Growth Kinetics
Motivation for studying cell growth kinetics
Cells are the biocatalyst. Different than enzymes, thecatalyst amount (cell) is changing during the catalyticprocess.Growth is a result of replication and changes in cell size.Cells extract nutrients from the environment and convertthem into biological products.Most products of interest will be associated with the growthand activity of cellsDesign of systems (e.g. system size and duration) requiresknowledge of  –Accumulation rate of cells –Depletion rate of nutrients –Accumulation rate of desired product
 
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Methods used to measure microbialgrowthDirect Method
 –Cell number 
Microscopic countsParticle countePlate count
 –Cell mass
Direct methodTurbidityDry weight
Indirect method
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Viable counts (Fig. 6.21, 6.22)
Each colony on plate or filter arises from single livecellOnly counting live cellsNeed to carefully select growth medium
 
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Microscopic counts (Fig. 6.23)
Need a microscope, special slides, high poweobjective lensStaining can be used to distinguish dead and livecellsSuits for bacteria and yeastsCell pass through theorifice and generate aelectric pulseWeight of pulsecorresponding to cellsizeSuitable for discretecells in a particle freemedium
Particle counter 

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