Asian J. Pharm. Res. 2012; Vol. 2: Issue 1, Pg 19-31
ISSN- 2231–5683 (Print)
ISSN- 2231–5691 (Online)0974-3618
Secondary Metabolite screening, Bioactive compound extraction, andDisrupting Mitotic Activity of Wild Cabbage [Brassicaceae] towardsCancer Management.
, Dr. U.S Mahadeva Rao
Assistant Professor, PG Department of Biochemistry, SRM Arts and Science College, Chennai-603203, India.
Associate Professor, Department of Biochem., Faculty of Medicine and Health Science, Universiti Sultan ZainalAbidin, Malaysia.*Corresponding Author E-mail:
Objective: The present study has been formulated with an objective to establish the preliminary phytochemicalanalysis and antimitotic activity of Brassica oleracea. Method: Brassica oleracea was collected, homogenized andextracted with different solvents. Antimitotic activity of Brassica oleracea was evaluated using the meristamatic cellsfrom the root of Allium cepa. Experiments were carried out with incorporation of folic acid in the extract. Results: Thepreliminary study revealed the presence of flavonoids, steroids, alkaloids, saponins, polyphenols and glycosides. Folicacid inhibited the antimitotic activity of Brassica oleracea extract. The antimitotic activity obtained was compared withmethotrexate-a referred anticancer drug. Discussion: The results obtained from the present study pinpoint thatantimitotic activity of Brassica oleracea may be due to the presence of flavonoids, steroids, alkaloids, polyphenols andsaponins. Conclusion: Hence Brassica oleracea is a promising source of phytochemicals which promote human healthby strengthening the human immune system, inactivate cancer-causing substances, protect the heart and eyes fromdisease, boost enzyme activity to increase the benefits of the various protective enzymes, reduce bad cholesterol levels,and anti-aging.
Antimitotic; Brassica oleracea; Allium cepa; Polyphenols; Saponins.
The “functional food” industry has produced and marketedfoods enriched with bioactive compounds, but there are nouniversally accepted criteria for judging efficacy of thecompounds or enriched foods. The lack of understandingbioactive compounds and their health benefits should notserve to reduce research interest but should insteadencourage plant and nutritional scientists to work togetherto develop strategies for improvement of health throughfood
.Cruciferous vegetables are an excellent dietary source of phytochemicals including glucosinolates (and glucosinolatebreakdown products), phenolics and other antioxidants likevitamins(C, K1, etc.), as well as dietary essential minerals(Ca, Mg, Na, K, Fe, Zn, etc.). Dietary antioxidants (i.e.vitamins, flavonoids) present in broccoli may decrease therisk of certain cancers
Received on 24.12.2011 Accepted on 10.02.2012© Asian Pharma Press All Right Reserved
Asian J. Pharm. Res. 2(1)
: Jan.-Mar. 2012; Page 19-31
Broccoli is a plant of the cabbage family
It is classified in the Italica cultivargroup of the species
. Broccoli has largeflower heads, usually green in color, arranged in a tree-likefashion on branches sprouting from a thick, edible stalk.The mass of flower heads is surrounded by leaves. Broccolimost closely resembles cauliflower, which is a differentcultivar group of the same species
vegetables has been focused on theedible parts. However, scarce information is availableregardingthe corresponding by-products, which are in fact agood source of phenolic compounds of this unusual foodproduct (i.e. cauliflower leaf as by-product) with possibleuses as a dietary or food antioxidant
.The cancer-protective properties of
(i.e. broccoli)consumption are most likely mediated through “bioactivecompounds” that induce a variety of physiological functionsincluding acting as direct or indirect antioxidants,regulating enzymes and controlling apoptosis and the cellcycle.