Metabolic Enzyme Regulation
* before regulator means enzyme is phosphorylated by a kinase (*PKA = Phosphorylated by Protein Kinase A)
* after regulator means enzyme is dephosphorylated
Ca2+ regulates by associating with calmodulin....Ca2+-Calmodulin complex mediates effects.
Assume regulation by PKA is part of the Glucagon pathway.
ENZYME/PROTEIN ACTIVATION INHIBITION COMMENTS
GLYCOLYSISHexokinaseGlucose-6-PGlucokinaseGlucose-6-POnly in Liver PFK-1AMPF-2,6-BPATPCitratePEPLow pHLow pH - such as inanaerob. glycolysis (lacticacid)PFK-2AMPPEPCitrate*PKAF-6-P <--> F-2,6-BP Not a GlycolyticEnzyme...Provides al-losteric effector Pyruvate KinaseF-1,6-BPATP*PKALDHLactateCori Cycle (liver)Lactate-->PyruvatePDHPyruvateCoA / NAD+ / ADPCa2+ / Mg2+Acetyl CoA NADHATPKNOW MECHANISMRequires TPP, LipoicAdic, FADRegulated by PDH Kinaseand PDH PhosphataseTCA CYLCLECitrate SynthaseCitrateIsocitrate DHADPCa2+ NADHa-Ketoglutarate DHCa2+NADH