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Table Of Contents

Chapter 1: Introduction
Chapter 2: Objectives and Hypothesis
Significance of Study
Specific Objectives
Research Hypotheses
Basic Assumptions
Chapter 3: Review of Literature
Type I Diabetes
Rodent Models of Type I Diabetes
Genetic Susceptibility
Environmental Factors
Viral Infections
Diet
Sphingolipids
Structure
Sphingolipids in Foods
Metabolism of Sphingolipids
Possible Mechanisms for Triggering Type I Diabetes by Sphingolipids
Cell-Regulation Functions of Sphingolipids
Disturbance of Gut Immune System by Sphingolipids
Chapter 4: Materials and Methods
Animals
Wheat Gluten
Chemicals
Extraction of Sphingolipid-Enriched Lipid Fraction
Isolation of Total Lipids
Fractionation of Total Lipid
Determination of Crude Protein
Determination of Crude Fat
Determination of Moisture
Determination of Ash
Determination of Carbohydrate
Detection of Lipids by Thin Layer Chromatography (TLC)
Detection of Glycolipids
Detection of Sphingolipids
Detection of Phospholipids
Detection of Glucosylceramide by HPLC
Detection of Glucosylceramide by Mass Spectrometry (MS)
BBdp Rats Feeding Study
Statistics
Chapter 5: Results and Discussion
Lipid Extraction:
Proximate Analysis
Detection of Lipids by TLC
Detection of glucosylceramide by HPLC
Body Weight over Time in Male BBdp Rats
Figure 7. Body weight over time in male BBdp rats
Body Weight over Time in Female BBdp Rats
Summary
Limitations
Suggestions for Future Research
Chapter 6: References
Appendices
Vita
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Published by: Camden White on Jan 17, 2013
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10/19/2013

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