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Evaluation of common protein extraction reagents in mammalian and bacteria lysates

Evaluation of common protein extraction reagents in mammalian and bacteria lysates

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Successful biomarker analysis requires optimizing the sample preparation method not only for the liberation of the marker but also for the downstream analytical method. Evaluation of several lysis buffers for the detection of breast cancer markers revealed that no single system was optimal for all samples. In one case, RIPA buffer generated higher “total protein” content when applied to the lysis of breast cancer tissue, while CytoBuster™ Extraction Reagent was more efficient for the liberation of the desired biomarker. Application of the Direct Detect™ spectrometer and SNAP i.d.® 2.0 system allowed fast turnaround of the experiments, enabling rapid screening and optimization of sample preparation.
Successful biomarker analysis requires optimizing the sample preparation method not only for the liberation of the marker but also for the downstream analytical method. Evaluation of several lysis buffers for the detection of breast cancer markers revealed that no single system was optimal for all samples. In one case, RIPA buffer generated higher “total protein” content when applied to the lysis of breast cancer tissue, while CytoBuster™ Extraction Reagent was more efficient for the liberation of the desired biomarker. Application of the Direct Detect™ spectrometer and SNAP i.d.® 2.0 system allowed fast turnaround of the experiments, enabling rapid screening and optimization of sample preparation.

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Categories:Types, Research
Published by: EMD Millipore Bioscience on Jan 19, 2013
Copyright:Attribution Non-commercial

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07/28/2013

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