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Biological applications of Pyoverdines and their complexes with Iron and Gallium

Biological applications of Pyoverdines and their complexes with Iron and Gallium

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Published by Sigma-Aldrich
A poster on Biological applications of Pyoverdines and their complexes with Iron and Gallium, presented by Sigma-Aldrich.
A poster on Biological applications of Pyoverdines and their complexes with Iron and Gallium, presented by Sigma-Aldrich.

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Categories:Types, Research, Science
Published by: Sigma-Aldrich on Feb 13, 2009
Copyright:Attribution Non-commercial

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06/16/2009

 
Abstract
Pyoverdines (pseudobactins) are fluorescent siderophores that have high-affinity for iron (10
-32
M). The fluorescent
Pseudomonas
group synthesizesthem under iron-deficient growth conditions. Pyoverdines are composed ofthree structural parts: a dihydroxyquinoline chromophore, a side chain and avariable peptidic chain. The peptide moiety is involved in receptor recognitionand binding. The size and amino acid composition of pyoverdines are uniqueto each species, as well as the pyoverdine recognition specificity. Despite thisspecificity, many fluorescent
 pseudomonads
are able to utilize pyoverdinesproduced by other strains.We have isolated three different siderophores of the pyoverdine type from
Pseudomonas fluorescens
. These pyoverdines, differing in their side chain,were identified by HPLC and mass spectra (m/z 1161, 1190 and 1160). Weshow that these three forms of pyoverdines, when complexed to iron, areeffective growth promoters for bacteria in a minimal medium. In contrast, amixture of these pyoverdines complexed to Gallium acts as a useful inhibitorof growth for both
Pseudomonas fluorescens
and
Pseudomonas aeruginosa
.In addition, these pyoverdines are effective in protecting
Escherichia coli 
fromthe deleterious effects of reactive oxygen intermediates (ROI). These resultsshed light on novel uses of pyoverdines and their complexes as ROI protectingand antibacterial agents.
Fermentation and Purification
25
0
C 30
0
C
 
25
0
C 30
0
C
P. fluorescens
was grown at 25ºC (left) and 30ºC (right).Only at 25ºC Pyoverdines were produced (fluorescent green).
Figure 1.
 
Pyoverdines production is temperature dependent
P. Fluorescens
5
liter fermentationThe green color is indicative of pyoverdines production
This fermentation was also performed in large scale fermentors
Figure 2.
Pyoverdines fermentation
Biological applications of Pyoverdines and their complexes with Iron and Gallium
Eliana Schlosser-Silverman, Marcia Ben-Shoshan, Shlomo Pleban*, Dorit ZharharyBiotechnology R&D Department, Sigma-Aldrich Israel
Components of the pyoverdines mixture.Each component is composed of three structural parts: Peptide chain, Chromophoreand Side chain.The three components of the mixture differ in their side chain.
Component Identification
Side chainm/z
-NH-CO-CH
2
-CH
2
-COOHSuccinic acid1161-NH-CO-CH
2
-CH
2
-CHOH-CONH
2
2-hydroxy glutaramide1190-NH-CO-CH
2
-CH
2
-CONH
2
Succinamide1160
Fermentation BrothTitration to Acidic pHBind to FeCl
3
.6H
2
O (Fe-Pv) or Ga(NO
3
)
3
. H
2
O (Ga-Pv)Chromatographic purification using gel filtrationPurified Compounds: Pv; Fe-Pv; Ga-Pv
Figure 3.
Purification of Pyoverdines (Pv) and their Iron and Gallium complexes
Fe-PvPvbasic pHPvacidic pHGa-Pv
 
Fe-PvPvbasic pHPvacidic pHGa-Pv
Figure 4.
 
Pyoverdines mixture components

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