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Published by Neeraja Vegesna

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Published by: Neeraja Vegesna on Jan 31, 2013
Copyright:Attribution Non-commercial


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Aqueous Two Phase System for the separationfor Bio molecules
The aqueous two-phase system is designed by a binodal curve whichseparates two-phase area from the single phase zone.
The phase diagram gives the exact composition of the top and bottom phasesystem
The composition represented by the points below the binodal curve arehomogenous
The two phases are formed only by the composition above the binodal curve
Tie line
The line joining points representing the top and bottom phase composition in the phase diagram iscalled tie line ( these points are not arbitrary points in phase diagram )
Any points in the same tie line will have same phase composition but of different in volume ratio of the phases
A tie line TMB was fitted with in the phase diagram
Point T( node point)
Top phase composition;Point B( node point)
Bottom Phase composition;Point M
Represent composition of phase system
Plate point ( critical point)
The point
represent critical point, which is point at which the composition and volumeof the two phases theoretically become equal.
System with a
short tie line
have phases whose composition differs less than those of a system on alonger tie line.System with
higher tie lines
, proteins as well as others partitioned substances will have an increasingly more one-sidedpartitioning towards on of the phases.This creates the possibility of adjusting the extraction via choice of tie line.

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