American Journal of Applied Sciences 9 (3): 337-342, 2012ISSN 1546-9239© 2012 Science Publications
Nobuyuki Hayashi, Department Applied Biochemical Food Science, Faculty of Agriculture,Saga University, Saga 840-8502, Japan Tel: 81-0952-288751
The Effects of Batch Reactor Extraction onAntioxidant Activity from
Siti Irma Rahmawati and
The United Graduate School of Agriculture Science,Agriculture Faculty, Kagoshima University, Japan
Department Applied Biochemical Food Science,Faculty of Agriculture, Saga University, Saga 840-8502, Japan
The main active compounds of benalu teh’s include alkaloids andflavonoids, therefore antioxidant of it has big potential to develop. In the context of extraction, thetraditional (boiling in the water until one-third remain) extraction require a long extraction time andresult in low yields of extraction also antioxidant activities.
Batch reactor is extraction athigh temperature under applied pressure to keep the solvent under liquid phase, by this technology wehope to obtain better results. Optimum extraction conditions of benalu teh need to be discovered toobtain the antioxidant inside it. Benalu teh was extracted using traditional and batch reactor withdifferent solvents (water, 30, 50, 70 and 99% ethanol) at different temperatures (40-180°C) fordifferent time periods (10-20 min) under additional pressure 0.2 MPa.
The best extractioncondition by batch reactor extraction was 30% ethanol solvent at temperature 100°C for 10 min.
Batch reactor extraction gave a better result than traditional extraction on extract yield,radical scavenging activities and total phenolic compound compared with the traditional extraction.
Extraction, benalu teh, flavonoid
Mistletoe is a semi-parasitic plant that is considersas an unwanted plant because attached to theeconomically important horticultural plant and reduceproduction. However on the other side, mistletoe hasbeen known as one of the medicinal plants used intraditional therapy. One of the mistletoe varieties oftenused in Indonesia as medicine is tea mistletoe. It’slocally known as Benalu Teh.The main active compounds in benalu tehinclude bioactive lipid, alkaloids, flavonoids andothers components (Ohashi
., 2003). Flavonoidsinside benalu teh consist of quercetin and itsglycoside, both of have strong antioxidant activity(Ikawati
., 2008).An antioxidant is a compound that has ability toinhibit oxidation rate or to neutralize a free radicals(Roberts, 2008). Oxidative damage is caused by freeradicals that may be related to aging and diseases,such as atherosclerosis, diabetes and cancer.Therefore, the antioxidant inside benalu teh has goodpotential as an antioxidant.In the context of extraction, the traditionalextraction method of boiling in the water until one-thirdof the solution remains which is not effective due totime spent and energy consumed. Differenttechnologies can be applied to obtain extracts with ahigh concentration of selected active principles.Moreover, solvents of various polarities andtemperatures can be utilized to adjust its selectivity(Valle
., 2005). In this research we used batchreactor extractor to keep the solvent in liquid phaseeven on high temperature by adding pressure higherthan vapor pressure. Through this technology we hopedto obtain better result than the traditional method.Ethanol, a polar solvent, effectively extractsflavonoids and their glycosides from raw materials(Bazykina
., 2002), but solubility of thesecompounds can be enhanced using a mixed solvent overa limit compositional range (Cacace and Mazz, 2003).Particularly, mixtures of alcohol and water haverevealed to be more efficient in extracting phenolicconstituent than the corresponding mono-componentsolvent system (Yilmaz and Toledo, 2006).Temperature and time of extraction are importantparameters to be optimized in order to minimize energy