Welcome to Scribd. Sign in or start your free trial to enjoy unlimited e-books, audiobooks & documents.Find out more
Download
Standard view
Full view
of .
Look up keyword
Like this
2Activity
0 of .
Results for:
No results containing your search query
P. 1
Mouse TNF-α ELISA Kit

Mouse TNF-α ELISA Kit

Ratings: (0)|Views: 28|Likes:
Published by Anogen

This Mouse TNF-alpha (α) ELISA Kit is to be used for the in vitro quantitative determination of mouse tumour necrosis factor alpha (mouse TNF-α) concentrations in serum and cell culture supernatant. This kit is intended FOR LABORATORY RESEARCH USE ONLY.

This Mouse TNF-alpha (α) ELISA Kit is to be used for the in vitro quantitative determination of mouse tumour necrosis factor alpha (mouse TNF-α) concentrations in serum and cell culture supernatant. This kit is intended FOR LABORATORY RESEARCH USE ONLY.

More info:

Categories:Types, Research
Published by: Anogen on Feb 11, 2013
Copyright:Attribution Non-commercial

Availability:

Read on Scribd mobile: iPhone, iPad and Android.
download as PDF, TXT or read online from Scribd
See more
See less

09/17/2013

pdf

text

original

 
S7.5 (01) mTNF-
α
 
Mouse TNF- α ELISA Kit
 
For the quantitative determination of mouse tumour necrosisfactor alpha (TNF-α) concentrations in mouse serum,cell culture supernatant
 Catalogue Number: MEC1003
96 tests
 
FOR LABORATORY RESEARCH USE ONLYNOT FOR USE IN DIAGNOSTIC PROCEDURES
 ANOGEN 
 
2355 Derry Road East, Unit 23Mississauga, OntarioCANADA L5S 1V6Tel: (905) 677-9221 or (877) 755-8324Fax: (905) 677-0023Email: info@anogen.ca
Web Site: www.anogen.ca
 
S7.5 (01) mTNF-
α
 
1
Page
 
TABLE OF CONTENTS
 
INTENDED USE 2INTRODUCTION 2PRINCIPLE OF THE ASSAY 2REAGENTS PROVIDED 4MATERIALS REQUIRED BUT NOT SUPPLIED 5PRECAUTIONS 5SAMPLE PREPARATION 6............
Collection, Handling and Storage
6PREPARATION OF REAGENTS 6 ASSAY PROCEDURE 7CALCULATION OF RESULTS 9TYPICAL DATA 9
.............Example one (Calibrator Diluent I)
 9
.............Example two (Calibrator Diluent II)
10PERFORMANCE CHARACTERICS 11
.............Intra-assay precision
11
.............Inter-assay precision
12
.............Recovery
12
.............Sensitivity
12REFERENCES 12
 
S7.5 (01) mTNF-
α
 
2
INTENDED USE
This Mouse TNF-alpha (α) ELISA Kit is to be used for the
in vitro
quantitativedetermination of mouse tumour necrosis factor alpha (mouse TNF-α) concentrations inserum and cell culture supernatant. This kit is intended FOR LABORATORY RESEARCHUSE ONLY.
INTRODUCTION
 Tumor Necrosis Factor alpha (TNF-α) is a multifunctional proinflammatory cytokine,mainly secreted by activated macrophages. The cytokine was named for its remarkableability to cause hemorrhagic necrosis of tumors in mice. It is implicated with a variety of biological procedures including systemic inflammation, cell proliferation, apoptosis, lipidmetabolism, and coagulation. It is well documented that TNF-
α
functions through itsreceptors, TNFR1 (p55) and TNFR2 (p75).TNF-
α
plays an important role in the immune response to bacterial, and certain fungal,viral, parasitic invasions, in tissue remodeling, autoimmune-diseases and the necrosis of specific tumors. The pleiotropic effect of TNF-
α
regulation is attributed to its ability totrigger multiple signaling pathways simultaneously. TNF-
α
hyper-expression in responseto the components of some bacteria such as LPS can cause life threatening septic shock.Recombinant TNF-α, in combination with chemotherapy, has been applied for synergistictreatment of soft sarcomas, melanomas and other irresectable tumors. Anti-TNF-
α
 therapy has been used for treatment of rheumatoid arthritis.
This mouse TNF-α ELISA is a 3.5 hour solid phase immunoassay readily applicable tomeasure mouse TNF-
α 
levels in serum, plasma, cell culture supernatant in the range of to 1000pg/mL.
PRINCIPLE OF THE ASSAY
This mouse TNF-α enzyme linked immunosorbent assay (ELISA) applies a techniquecalled a quantitative sandwich immunoassay. The microtiter plate provided in this kit hasbeen pre-coated with a monoclonal antibody specific to mouse TNF-α. Standards or samplesare then added to the appropriate microtiter plate wells with a biotin-conjugatedmonoclonal antibody preparation specific for mouse TNF-
α
and incubated. Mouse TNF-
α
 if present, will bind and become immobilized by the antibody pre-coated on the wells andthen be “sandwiched” by biotin conjugate. The microtiter plate wells are thoroughlywashed to remove unbound mouse TNF-α and other components of the sample. In order to quantify the amount of mouse TNF-α present in the sample, avidin conjugated toHorseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is

You're Reading a Free Preview

Download
scribd
/*********** DO NOT ALTER ANYTHING BELOW THIS LINE ! ************/ var s_code=s.t();if(s_code)document.write(s_code)//-->