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Edexcel GCE Biology Unit 3B - QP

Edexcel GCE Biology Unit 3B - QP

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6BI07 - Question Paper
6BI07 - Question Paper

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Published by: Ahmed Shaffan Mohamed on Feb 22, 2013
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04/02/2013

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Centre NumberCandidate NumberWrite your name here
SurnameOther names
 Total Marks
Paper Reference
Turn over 
P39882A
©2013 Pearson Education Ltd.
1/1/1/1/
*P39882A0116*
Edexcel GCE
Biology
Advanced SubsidiaryUnit 3B: Practical Biology and Research Skills
Monday 7 January 2013 – Afternoon
Time: 1 hour 30 minutes
6BI07/01
You must have:
Ruler, Calculator, HB pencil
Instructions
 
Use
black 
ink or ball-point pen.
 
Fill in the boxes
at the top of this page with your name,centre number and candidate number.
 
Answer
all
questions.
 
Answer the questions in the spaces provided
there may be more space than you need 
.
Information
The total mark for this paper is 40.
The marks for
each
question are shown in brackets
– use this as a guide as to how much time to spend on each question.
Advice
 
Read each question carefully before you start to answer it.
Keep an eye on the time.
Try to answer every question.
Check your answers if you have time at the end.
 
2
*P39882A0216*
BLANK PAGE
 
3
*P39882A0316*
Turn over 
Answer ALL questions.1
In a class practical, a student had used tissue culture techniques.He decided to investigate the conditions needed for the growth of plant cells. Plantcells can grow to form a callus, a mass of undifferentiated cells.The main ingredients needed for the growth of calluses are: 
mineral salts 
auxin (IAA) 
cytokinin 
sugar, such as sucrose or glucose.The student decided to investigate the effect of glucose concentration on the growthof calluses from pieces of cotton plant tissue.He mixed the ingredients listed above with an agar solution to make a gel on whichhe could grow the calluses. Working in sterile conditions, he removed small pieces of tissue, known as explants, from one cotton plant. The explants were placed on thisgel.This procedure was repeated using agar gel containing different concentrations of glucose.After two weeks, he recorded the percentage of explants that had formed callusesand the dry mass of the callus.(a) (i) Name
one
of the dependent variables in this investigation.
(1)
....................................................................................................................................................................................................................................................................................
(ii) Name the independent variable in this investigation.
(1)
....................................................................................................................................................................................................................................................................................

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