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Protein Chemistry
Postgraduate students’ course
 
CONTENTS
Chapter 1 Introduction to Protein Chemistry
1.1Overview—Advances in Protein Chemistry1.2General Concept of Protein1.3Protein Functions1.4Protein Structures1.4.1 The Building Blocks of Proteins1.4.2 Polypeptide Chain1.4.3 Conformation of Polypeptide Chain1.4.4 Protein Structure Biology1.5Interactions of Protein with Other Molecules1.6Protein Engineering1.7Proteome and Proteomics1.8International Journals and Websites Related toProtein Chemistry
Chapter 2 Physic-chemical Properties of Proteins
2.1 Size and Shape of Protein Molecules2.2 The Charge Properties of Protein Molecules2.2.1 The Charge Properties of Amino Acids2.2.2 The Charge Properties of Protein Molecules2.2.3 Isoelectric Point of Proteins2.2.4 Electrophoresis2.2.5 Ion Exchange2.3 Colloid Property of Proteins2.3.1 Diffusion2.3.2 Viscosity2.3.3 Impermeability
 
2.4 Precipitation2.4.1 Salting out and Salting in2.4.2 Basic and Acidic precipitation2.4.3 Organic Solvent-caused precipitation2.4.4 Heavy metal-caused precipitation2.4.5 Interaction of Antibody and Antigen2.4.6 Others2.5 Color-formation of Proteins2.6 Spectro-properties of Proteins2.6.1 Ultraviolet absorption2.6.2 Fluorescence spectrum2.6.3 Circular Dichroism Spectrum
Chapter 3 Methods for Characterization andPurification of Proteins
3.1 Methods of Protein Characterization3.1.1 Solubility Reflects a Balance of Protein-SolventInteraction3.1.2 Several Methods are Available for Determina-tion of Gross Size and Shape3.1.3 Electrophoretic Methods are the Best Way toAnalyze Mixtures3.2 Methods for Protein Purification3.2.1 Differential Centrifugation Subdivides CrudeExtracts into Two or More Fractions3.2.2 Differential Precipitation is Based on SolubilityDifferences
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