Connective Tissue Laboratory

Disusun oleh

MUHAMMAD ALIF PAKUBUANA 702012041 Fakultas Kedokteran UNIVERSITAS MUHAMMADIYAH PALEMBANG TAHUN AJARAN 2012/2013

Mast Cells

Blood Vessels

CT spread - low magnification Identify:

1. Mast cells 2. Blood vessels.

Mast Cells Elastic Tissue Fibers

Extracelluler Matrix

CT spread -higher mag.

Identify:
1. Mast cells 2. Elastic tissue fibers. 3. Extracellular matrix

Blood Vessel

Elastic Fibers

Mast Cells

Macrophage

CT spread - high mag.

Identify:
1. Mast cells 2. Macrophage 3. Blood vessel 4. Elastic fibers

Macrophages

Elastic Fibers

CT spread - med. mag.

Identify:
1. Macrophages 2. Elastic fibers

Epithelium

Loose Irregular Connective Tissue - Lamina Propria

muscle

Colon in x-section med. mag.

Identify:
1. Epithelium 2. Loose irregular connective tissue - lamina propria.

Connective Tissue

Epithelial Lined Glands

Colon -higher mag.

Identify:
1. Connective tissue 2. Epithelial lined glands.

Macrophage

Plasma Cells

Fibroblasts

Small Lymphocytes

Colon -high mag.

Identify:
Plasma cells Small lymphocytes Fibroblasts Macrophage

Epithelial Lined Villi

Loose Irregular Connective Tissue Of The Lamina Propria

Duodenum - medium mag.

Identify:
1. Epithelial-lined villi 2. Loose irregular connective tissue of the lamina propria

The pink tissue on the right is smooth muscle (m).

Nuclei Of Fibroblasts Endothelial Cells

Lymphocytes

Plasma Cells

Red Blood Cells

Duodenum - high mag.

Identify:
1. Plasma cells 2. Lymphocytes, 3. Nuclei of fibroblasts, 4. Endothelial cells 5. Red blood cells

Connective Tissue Of The Lamina Propria Green Staining Collagen Fibers

Esophagus - trichrome stain

Identify:
1.Connective tissue of the lamina propria, 2. Green staining collagen fibers.

Coarse (Green) Collagen Bundles

Fibroblast Nuclei

Esophagus high mag.

Identify:
1. Coarse (green) collagen bundles 2. Fibroblast nuclei

Stratified Squamous Epithelium

low Skin
Connective Tissue

Identify: 1. Stratified squamous epithelium 2. Loose irregular connectivetissue 3. Dense irregular fibroelastic 4. Connective tissue 5. Coarse collagen bundles

med

Dense Irregular Connective Tissue

Spleen - low and high mag

Identify:
1. Dense irregular connective tissue

Muscle-tendon junction - low power.

Identify:
1. Dense, regularly arranged, connective tissue - tendon. 2. Collagen fibers Dense, regularly arranged, 3. Fibroblast nuclei connective tissue - tendon

Fibroblast Nuclei

Collagen Fibers

Fibroblasts

Collagen Fibers

Tendon

Muscle-tendon junction- high mag.

Identify:
1. Tendon, 2. Collagen fibers 3. Fibroblasts

Collagen Bundles

Loose Connective Tissue

Fibroblast Nuclei

Tendon- medium mag.

Identify:
1. Collagen bundles 2. Fibroblast nuclei 3. Loose connective tissue

Tendon- high mag.

Identify:
1. Fibroblast nuclei 2. Collagen fibers.

Collagen Fibers

Fibroblast Nuclei

Spleen - medium mag.

Identify:
1. Reticular fibers 2. Loose connective tissue

Loose Connective Tissue

bv

Reticular Fibers

The Stroma Of The Lymph Node

Lymph node - med. mag.

Identify:
1.The stroma of the lymph node

Dense Irregular Connective Tissue Capsule

Fibroblasts

Macrophages

Small Lymphocytes

Lymph node -high mag.

Identify:
1. Dense irregular connective tissue capsule 2. Fibroblasts 3. Small lymphocytes 4. Macrophages.

Lymphocytes

Macrophages

Plasma Cells

Lymph node - high mag.

Identify:
1. Plasma cells 2. Lymphocytes; 3. Macrophages

Adipose Tissue

Mammary gland, adipose tissue - medium mag.

Identify: 1. Adipose tissue

Nerve (X&L of Sec.) - med. mag.

Identify 1. Fat cells

Fat Cells

Nerve (cross section) - high mag.

Identify:
1. A fat cell

A Fat Cell

PR EPITHEL

Epithelium Laboratory

Kidney, Simple squamous epithelium - low mag.

Cortico-medullary junction is identified by the red dotted line. The cortex (C) contains renal corpuscles surrounded by tubules of the nephron. The medulla (M) contains tubules and blood vessels are found throughout the cortex and the medulla. At higher magnification the tubules and the renal corpuscles of the cortex can be seen (next slide).

Simple Squamous Epithelium With Flattened Nuclei

Simple Squamous Epithelium (Endothelium)

Kidney, renal corpuscle, simple squamous epithelium. - med. mag.

Identify:
1. Simple squamous epithelium (endothelium) 2. Simple squamous epithelium with flattened nuclei.

Nuclei Of The Simple Squamous Epithelium

Simple Cuboidal Epithelium

Brush Borders Of Microvilli

Kidney, renal corpuscle, Simple squamous epithelium. - High mag.

Identify:
1. Nuclei of the simple squamous epithelium, 2. Simple cuboidal epithelium. 3. Brush borders of microvilli.

Basal Infoldings Of The Plasma Membrane Columnar Cells And Their Nuclei Nuclei

Parotid gland, Simple columnar epi. - low & high mag.

Identify:
1. Columnar cells and their nuclei nuclei 2. Basal infoldings of the plasma membrane.

Simple Columnar Epithelium With Their Nuclei Pale-Staining Mucus

Esophagus/Stomach junct., Simple columnar epi

Identify:
1. Simple columnar epithelium with their nuclei 2. Pale-staining mucus.

Goblet Cells

Absorptive Cells

A Simple Columnar Epithelium

low Duodenum, Simple columnar epithelium, - low & med. mag.

Identify:
1. A simple columnar epithelium 2. Goblet cells 3. Absorptive cells.

Simple Columnar Epithelial Cells Goblet And Absorptive Cells The Brush Border Composed Of Microvilli The Terminal Web

Duodenum, villus, simple columnar epithelium, - high mag.

Identify:
1. Simple columnar epithelial cells. 2. Goblet & absorptive cells. 3. The brush border composed of microvilli. 4. The terminal web.

Ciliated Cells

A Simple Columnar Epithelium

Secretory Cells Cilia

Uterine tube, Simple columnar epi., - Low, med. & high mag.
Identify:
1. A simple columnar epithelium. 2. Ciliated cells 3. Secretory cells. 4. Cilia

Thin skin, stratified cuboidal epi. (low med. & high mag.
A Stratified Cuboidal Epithelium

Identify:
1. A stratified cuboidal epithelium

A Stretified Columnar Epithelium

Sublingual gland, Stratified columnar epi.,-low & med. mag Identify:

1. A stratified columnar epithelium.

A Stratified Epithelium

Sublingual gland, Stratified columnar epithelium, - high mag. Identify:

1. A stratified epithelium.

A Stretified Squamous Non-Cornified Epithelium

Esophagus, (wet) stratified squamous epithelium, -low & high mag. The Squamous Cells Nuclei
Identify:
1. A stratified squamous non-cornified epithelium. 2. The squamous cells nuclei

A Thick, Non-Keratinized Epithalium

Lip, Stratified squamous epithelium (wet), low & high mag. Identify:
1. A thick, non-keratinized epithelium

Melanin

The Keratinized Stratified Squamous Epithelium And Its Basal Layer

Thin skin, stratified squamous epi.(cornified).- low & med. mag.

Identify:
1. The keratinized stratified squamous epithelium and its basal layer 2. Melanin

Keratinized Cells At The Surface

The Basal Cells Of The Stratified Squamous Epithelium

Thick skin, stratified squamous epi (cornified)-low & med. mag.

Identify:
1. The basal cells of the stratified squamous epithelium, 2. Keratinized cells at the surface.

The Transitional Epithelium

The Pear-Shaped To Cuboidal Most Superficial Cells

Bladder, transitional epithelium, - high mag.

Identify:
1. The transitional epithelium 2. The pear-shaped to cuboidal most superficial cells

The Pseudostratified Ciliated Epithelium

Trachea, pseudostratified columnar epithelium, low & med.mag. Identif

1. The pseudostratified ciliated epithelium

Columnar Cells

Ciliated Cells

Goblet Cells

Basal Cells

2-3 Rows Of Nuclei

Trachea, pseudostratified ciliated epithelium, - high mag.

Identify:
1. 2 - 3 rows of nuclei, 2. Basal cells 3. Columnar cells. 4. Goblet cells. 5. Ciliated cells.

Basal Cells

Long Microvilli (Streocilia)

Tall Columnar Cells

Epididymus, pseudostratified columnar epi, low & high mag.

Identify:
1. Basal cells 2. Tall columnar cells 3. Long microvilli (stereocilia)

PR BLOK 4

Muscle
Laboratory

Duodenum low & medium mag.

Identify:
1. Smooth muscle cells in the outer longitudinal layer and those in the inner circular layer.

Artery Low mag.

Identify:
1. The smooth muscle cells 2. The connective tissue matrix.

The Smooth Muscle Cells

The Connective Tissue Matrix

Connective Tissue Smooth Muscle

Anal Canal - med. & high mag.

Identify:
1. smooth muscle 2. connective tissue

Connective Tissue

Smooth Muscle

Uterine Tube - low. & med. mag.

Identify:
1. Smooth muscle 2.Connective tissue

 Esophagus

low & high mag

Identify:
1. Smooth muscle 2. Skeletal muscle.