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Kardiologia Polska 2007; 65: 4
Effect of supervised integrated exercise on heart rate variability in type2 diabetes mellitus
Shreedhar Bhagyalakshmi
1
, Haleagrahara Nagaraja
2
, BangraAnupama
3
, Bhat Ramesh
1
, Adhikari Prabha
4
,Murthy Niranjan
1
, Avabratha Shreedhara
5
1
Department of Physiology, Kasturba Medical College, Mangalore, India
2
Faculty of Medicine, International Medical University, Kuala Lumpur, Malaysia
3
Faculty of Medicine, University College Sedaya International, Kuala Lumpur, Malaysia
4
Department of Medicine, Kasturba Medical College, Mangalore, India
5
Department of Paediatrics, Kasturba Medical College, Mangalore, India
 
Original article
Address for correspondence:
HS NagarajaPhD, Department of Human Biology, International Medical University (IMU), Plaza Komanwel, Bukit Jalil, Kuala Lumpur,Malaysia, tel.:60386567228, fax:60386567229, e-mail: hsnagaraja@rediffmail.comRecei
 
ved:12 June2006.Ac
 
cepte
 
d:03 January2007.
 
Abstract
Background:Heart rate variability (HRV) reflects autonomic nervous system modulation of cardiac activity. There isarelationship between degrees of physical activity, HRV changes and the risk of cardiovascular disease.Aim:To study the effect of asupervised integrated exercise programme on HRV in type2 diabetes mellitus (DM).Methods:The study group consisted of48 patients (27 males, mean age62±7years) with type2 diabetes, of whom28underwent aspecial exercise programme whereas the remaining20 did not and served as the control group. The supervisedintegrated exercise programme was applied for aperiod of9 months. Deep breathing time domain HRV (difference betweenthe shortest and the longest R-R interval over one minute) was measured at baseline and after3,6 and9 months.Results:Asignificant improvement in the HRV values was observed with increasing duration of exercise (13.03±1.08beats/min at baseline versus16.5±1.11 beats/min at9 months, p <0.001) whereas HRV decreased in the control group(14.85±1.15 beats/min at baseline vs.14.30±1.75 at9 months, p <0.05). Favourable changes in HRV in the exercise group weregender-dependent and were significant in males (12.4±1.76 beats/min at baseline vs.16.18±1.91 at9 months, p <0.001) whereasin females only atrend towards HRV improvement was observed. The HRV changes were also age-dependent and were morepronounced in younger patients than in the elderly. The metabolic parameters of diabetes control (blood glucose andglycosylated haemoglobin levels) significantly improved in the exercise group and significantly worsened in the control group.Conclusions:Regular supervised integrated exercise significantly improves HRV in patients with type2DM, which mayfavourably influence their long-term prognosis.Key words:diabetes, heart rate variability, exerciseKardiol Pol2007;65: 363-368
Introduction
Heart rate variability (HRV) is anon-invasiveelectrocardiographic marker, reflecting the effects of the autonomic nervous system (ANS) on the sinus nodeof the heart [1]. Heart rate variability expresses the totalamount of variations of both instantaneous heart rate(HR) and R-R intervals. Heart rate variability hasrecently become apopular non-invasive research tool incardiology. Recent studies have shown that decreasedfluctuation of R-R intervals is not noise, but implicatesan increased risk of arrhythmic events and an increasedmortality rate in patients with aprevious myocardialinfarction (MI) [2]. Time and frequency domainmeasures of HRV have provided prognostic informationand also made it possible to perform non-invasivestudies on the significance of changes in the regulationof HR behaviour.
 
Kardiologia Polska 2007; 65: 4
In population studies decreased HRV has been of predictive value for mortality among healthy adults [3].Among the diverse conditions associated with decreasein HRV areMI, congestive heart failure (CHF) anddiabetes mellitus (DM) [4]. Several studies indicatedthat diabetic patients have reduced HRV [4-7].Abnormal HRV in diabetes represents an increased riskfor ventricular arrhythmias, as well as totalcardiovascular morbidity and mortality [8].Although data on the effects of physical training onautonomic control in healthy subjects remaincontroversial, the mortality reduction observed inphysically active subjects compared with sedentarypersons strongly suggests that exercise is beneficial.Individuals who engage in regular physical activity havealower prevalence of cardiovascular risk factors.Accordingly, exercise is considered an importantadjuvant therapy in risk factor modification [9]. Hence,there is still aneed to demonstrate to the communityand physicians the numerous benefits of regularphysical training. Regular exercise training is capable of modifying the autonomic balance. Recent studiesshowed that even asingle bout of maximal exercise isable to positively affect the autonomic balance of normal subjects for up to24 h. Longitudinal studieshave shown that exercise training increases HRV invarious conditions such as coronary artery disease(CAD) [10], acuteMI [11], cardiac rehabilitation patients[12], in patients on haemodialysis [13] and in healthyyoung and older adults [14]. However, very fewprospective studies have assessed the effects of exercise training on HRV in type2 diabetes patients.Hence the present research work was taken up withthe aim of studying the effects of integrated exerciseson HRV in patients with type2 diabetes mellitus. Ourresearch hypothesis was that integrated exercisetraining might increase HRV in diabetic patients.
Methods
Fifty-five type2DM patients who attendedadiabetes camp held in the hospital were included inthis study. Patients were interviewed and then invitedfor abaseline clinical examination. General physicalexamination, including height, body weight, waist-hipratio, blood pressure, pulse rate, respiratory rate andcomplete systemic examination, was performed.Detailed examination for pulmonary vascular disease,cerebral vascular disease and CAD was also done.
Measurements
Body weight and height were measured by standardmethods. A12-lead ECG was recorded and findings werenoted down. Blood was drawn from the antecubital veinof the seated patients and from the blood samples fastingblood glucose was measured by glucose dehydrogenasemethod. Glycosylated haemoglobin (HbA
1C
) was assessedby the immunoterbidimetric method.Diabetes mellitus patients were classified as havingdiabetes on the basis of history, regardless of durationof disease or need for anti-diabetic agents. Diabeteswas defined as fasting glucose
7.0 mmol/l. Diabetesmellitus patients with CHF, atrial fibrillation, frequentectopic beats, unstable angina, patients withMI andthose diabetes patients who were unable to performdeep breathing test were excluded from this study.From55 diabetic patients, twenty-eight wereenrolled for the exercise programme, which wasconducted under the guidance of aphysiotherapist.All28 diabetes patients were requested to visit thehospital regularly for5 days per week and practice theexercise daily.
Exercise programme
The training programme included:
warming up exercise for5 minutes,
cycling or treadmill exercise, depending on theexercise capacity of the patients for30 minutes,
cooling down exercise for10 minutes.The entire duration of the exercise did notexceed45 minutes for each session on each day.Twenty diabetes mellitus patients were recruited asanon-exercised group and served as controls. Thepharmacological treatment did not differ significantlybetween the two groups at baseline and there were nomajor changes during the study.Seven patients were withdrawn during the studyfor various reasons. In all,48 patients completed thestudy:20 controls and28 in the exercised group. Aninformed consent was obtained from all patientsbefore enrolling in the exercise programme and thestudy received the approval of the Institutional EthicalCommittee.
Heart rate variability analysis
Deep breathing HR test [15] was conducted inasupine positionduringstandard ECG recording.Before beginning the test, the subjects were taught tobreathe at arate of6 respiratory cycles per minute,5sfor each inhalation and5 s for each exhalation.Electrocardiogram was recorded continuously ataspeed of25 mm/s for60 s while the patientsbreathed as instructed.Beat-to-beat alterations in HR were evaluated by thetime domain method. The HRV interval (R-R intervals364
Shreedhar Bhagyalakshmi et al.
 
Kardiologia Polska 2007; 65: 4
between adjacent QRS complexes resulting from sinusnode depolarisation) was measured manually withascaled calliper. The R-R interval was measured in eachrespiratory cycle and an average R-R interval was takenfor the measurement of HRV. The variability in HR wascalculated as the difference between the shortest andlongest R-R intervals and expressed in beats/min. Eachpatient in the exercise study group was examined everymonth on aregular basis.During the follow-up, blood pressure, fasting bloodglucose, post-prandial blood glucose and body weightwere measured. The study was continued for aperiodof9 months. The follow-up HRV in the control groupwas also measured at the end of9 months.
Statistical analysis
The results are presented as mean±standarddeviation or numbers and percentages. For demo-graphics,Fisher’s test was employed to observedifferences in gender. The Mann-Whitney Utest wasemployed to compareparameters in theexercised andnon-exercised groups. The Wilcoxon signed rank testwas employed for statistical comparison between thebaseline and follow-up measurements of blood glucose,HbA
1C
and HRV. Astepwise multiple regression analysiswas used to determine the main factors influencing HRV.Ap value was taken as significant at5percent confidencelevel (p <0.05). The SPSS statistical package was used.
Results
The demographic characteristics of the patients aregiven in TableI. Comparison between the exercised andcontrol groups for various parameters usingMann-Whitney Utest showed that the two studygroups were well balanced and individuals in bothgroups did not differ significantly in age, gender,duration of diabetes, blood pressure, medication orsmoking status.As indicated by the Wilcoxon signed rank test, therewas no significant difference in the blood glucose levelin both exercise and control groups at the baselinerecordings (Table II). However, after9 months of theexercise programme, asignificant decrease in bloodglucose level (p <0.001) was seen compared to thebaseline levels. The blood glucose level increasedsignificantly (p <0.001) after9 months in the controlpatients. The patients who underwent the exerciseprogramme had significantly lower blood glucose levels(p <0.001) than control patients after9 months of thefollow-up period (Table II).Glycosylated haemoglobin level in the exercisedgroup was significantly lower (p <0.001) than thecontrol group at the start of the study. After9 monthsof integrated exercise, HbA
1C
level decreasedsignificantly (p <0.001) compared with the baselinevalues and was significantly lower than incontrols(p <0.001). In the control group, there was asignificantincrease in HbA
1C
after9 months of follow-up (Table II).Baseline HRV was significantly lower (p <0.001) in theexercised group than in controls. Asignificantimprovement in HRV (p <0.001) was observed in theexercised group after regular practice of supervisedintegrated exercise after9 months (Table II). Comparisonof the follow-up HRV between the exercise group andcontrols showed that there was asignificant (p<0.001)improvement in HRV in those who underwent thetraining programme. In the control group, asignificantdecrease in HRV (p <0.05) after9 months was found.
Exercise and heart rate variability in diabetes
365
ParameterControl Exercise
 
d p <groupgroupGender (M/F)20 (10/10)28 (22/6)0.062Age [years]59.45±2.7561.78±3.100.419Duration of diabetes[years]7.00±1.448.89±1.280.063Diabetes treatment [%]insulin360.716sulphonylureas13191.00other drugs340.351Hypertension6120.408Hypotensive agents 860.516Smoking460.905
 
Table I.Demographic and clinical characteristics
 
Parameter
 
sControl groupExercise
 
d groupBlood glucose [mmol/l]baseline10.68±0.6911.21±1.21after 9 months11.51±1.23
♦♦
9.52±1.02*
♦♦
HbA
1C
[%]baseline8.64±0.648.58±0.42
♦♦
after 9 months8.93±0.44
7.18±0.25*
♦♦
HRV [beats/min]baseline14.85±1.1513.03±1.08*after 9 months14.30±1.75
16.50±1.11*
♦♦
 
Table II.Glucose, HbA
1C
level and HRV valuesbefore and after exercise programme
 
*p<0.001 – exercise group vs. non-exercise group,
 p <0.05;
♦♦
 p <0.001 – baseline vs. 9 months follow-up in exercise and non-exercise group
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