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\u5357\u4eac\u533b\u79d1\u5927\u5b66\u9644\u5c5e\u5357\u4eac\u7b2c\u4e00\u533b\u9662
\u4e2d\u5fc3\u5b9e\u9a8c\u5ba4
Central Laboratory Of Nanjing First
Hospital Affiliated To Nanjing
Medical University
\u9020\u8840\u5e72/\u7956\u7ec6\u80de\u8ba1\u6570
\uff08CD34+\uff09\u64cd\u4f5c\u7a0b\u5e8f
\u7f16 \u53f7\uff1aSop-CL-FCM-007
\u65e5 \u671f\uff1a2009-03-05
\u7248 \u672c\uff1a\u7b2c\u4e00\u7248
\u7b2c1 \u9875
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\u62a5\u7ecf\u4e0b\u8ff0\u4eba\u5458\u6279\u51c6\u7b7e\u5b57\uff1a\u8d28\u91cf\u4e3b\u7ba1\u3001\u6388\u6743\u4eba\uff08\u79d1\u4e3b\u4efb\uff09\uff0c\u65b9\u53ef\u6539\u52a8\u3002
\u4e09\u3001\u9002\u7528\u8303\u56f4\uff1a
\u5916\u5468\u8840\u6216\u9aa8\u9ad3\u6db2\u4e2d\u9020\u8840\u5e72/\u7956\u7ec6\u80de\u8ba1\u6570\u3002
\u56db\u3001 \u6eaf\u6e90\uff1a
\u7f8e\u56fd BD \u516c\u53f8 ProCOUNT Progenitor Cell Enumeration Kit \u4ea7\u54c1\u8bf4\u660e\u4e66
(CAT:340498)\u3002
\u4e94\u3001\u5b9e\u9a8c\u539f\u7406\uff1a

ProCOUNT \u65b9\u6cd5\u6839\u636e\u7956\u7ec6\u80de\u7684\u7279\u5f81\uff0c\u4f4e SSC\u3001CD45 \u5f31\u9633\u6027\u3001\u6838\u9178\u67d3\u6599\u9633\u6027\u53ca CD34 \u9633\u6027\uff1b\u91c7\u7528\u6838\u9178\u67d3\u6599/CD34/CD45 \u67d3\u8272\u65b9\u6cd5\uff0c\u7279\u5f02\u8bc6\u522b\u6837\u672c\u4e2d\u5e72/\u7956\u7ec6\u80de\uff0c \u540c\u65f6\uff0cProCOUNT \u5b9e\u9a8c\u52a0\u5165 TRUCOUNT \u7edd\u5bf9\u503c\u8ba1\u6570\u7ba1\u4f5c\u5b9a\u91cf\u5206\u6790\uff0c\u8ba1\u7b97 CD34+\u7ec6\u80de cell/ul\uff0c\u4ece\u800c\u907f\u514d\u4e86\u8840\u7403\u8ba1\u6570\u4eea\u53ef\u80fd\u5e26\u6765\u7684\u8bef\u5dee\u3002

\u516d\u3001\u4e3b\u8981\u8bd5\u5242\uff1a

ProCOUNT Progenitor Cell Enumeration Kit (\u7f8e\u56fd BD Biosciences \u516c\u53f8\uff1b
CAT:340498)\uff0c
\u5176\u4e2d\u5305\u62ec\uff1a1\u3001\u6838\u9178\u67d3\u6599/CD34-PE/CD45-PreCP

2\u3001\u6838\u9178\u67d3\u6599/IgG1-PE/CD45-PreCP

3\u3001TruCOUNT Tubes
\u4e03\u3001\u5b9e\u9a8c\u64cd\u4f5c\uff1a
1\u3001\u6807\u672c\u91c7\u96c6\uff1a\u4f7f\u7528 EDTA-K2\u6297\u51dd\u771f\u7a7a\u91c7\u8840\u7ba1\uff0c\u62bd\u53d6\u9759\u8109\u8840 2ml\uff1b\u6216\u65b0\u9c9c\u809d\u7d20

\u6297\u51dd\u9aa8\u9ad3\u3002
2\u3001\u67d3\u8272\u548c\u56fa\u5b9a\u7ec6\u80de\uff1a
\uff081\uff09\u6807\u672c\u7ba1\u7f16\u53f7 A\uff0c\u53e6\u53d6 TruCOUNT \u7ba1\u4e24\u53ea AT\u3001AC\u3002
\uff082\uff09AT \u7ba1\u52a0\u5165\u6838\u9178\u67d3\u6599/CD34-PE/CD45-PreCP 20ul\u3002AC \u7ba1\u52a0\u5165\u6838\u9178\u67d3\u6599
/IgG1-PE/CD45-PreCP 20ul\u3002

\uff083\uff09AT\u3001AC \u7ba1\u4e2d\u7cbe\u786e\u52a0\u5165\u5168\u8840 50Ul\uff0c\u6df7\u5747\u3002
\uff084\uff09\u7f6e\u5ba4\u6e29\uff0c\u907f\u5149\u5b75\u80b2 15min\u3002
\uff085\uff09\u52a0\u5165 FACSLysing \u6eb6\u8840\u6db2 0.5ml\uff0c\u7f6e\u5ba4\u6e29\uff0c\u907f\u5149\u5b75\u80b2 30min\u3002

3\u3001\u6df7\u5300\u540e\u4e0a\u673a\u68c0\u6d4b\uff1b\u4eea\u5668\u81ea\u52a8\u5206\u6790\u7ed3\u679c\uff1b\u6253\u5370\u5b9e\u9a8c\u62a5\u544a\u3002

\u516b\u3001\u53c2\u8003\u503c\uff1a
\u5916\u5468\u8840 CD34+\u7ec6\u80de\uff1a0.01-0.1%
\u9aa8\u9ad3\u4e2d CD34+\u7ec6\u80de\uff1a0.05-0.5%

\u4e5d\u3001\u4e34\u5e8a\u610f\u4e49\uff1a

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\u547d\u3002\u9020\u8840\u5e72\u7ec6\u80de\u8ba1\u6570\u53ef\u4ee5\u5224\u65ad\u52a8\u5458\u6548\u679c\uff0c\u638c\u63e1\u5e72\u7ec6\u80de\u91c7\u96c6\u65f6\u673a\u3002\u4e5f\u53ef\u901a\u8fc7\u68c0
\u6d4b\u91c7\u96c6\u7269\u4e2d CD34+\u7ec6\u80de\u542b\u91cf\uff0c\u786e\u5b9a\u56de\u8f93\u7684\u91cf\uff0c\u901a\u5e38\u8981\u6c42 CD34+\u7ec6\u80de\u56de\u8f93\u91cf
\u5e94\u8fbe\u52302*106/KG \u4f53\u91cd\u3002
\u5341\u3001\u8d28\u91cf\u63a7\u5236\uff1a
1\u3001\u91c7\u7528\u514d\u6d17\u65b9\u6cd5\uff0c\u907f\u514d\u4e86\u6d17\u6da4\u8fc7\u7a0b\u4e2d CD34+\u7ec6\u80de\u7684\u4e22\u5931\u3002
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