MEETING ABSTRACT Open Access
The neuroprotective effects of electrolyzedreduced water and its model water containingmolecular hydrogen and Pt nanoparticles
, Taichi Kashiwaki
, Takeki Hamasaki
, Tomoya Kinjo
, Kiichiro Teruya
, Shigeru Kabayama
22nd European Society for Animal Cell Technology (ESACT) Meeting on Cell Based TechnologiesVienna, Austria. 15-18 May 2011
Human brain is the biggest energy consuming tissue inhuman body. Although it only represents 2% of thebody weight, it receives 20% of total body oxygen con-sumption and 25% of total body glucose utilization. Forthat reason, brain is considered to be the most vulner-able part of human body against the reactive oxygenspecies (ROS), a by-product of aerobic respiration. Oxi-dative stress is directly related to a series of brain dys-functional disease such as Alzheimer
s disease etc. Electrolyzed reduced water(ERW) is a functional drinking water containing a lot of molecular hydrogen and a small amount of platinumnanoparticles (Pt NPs, Table1). ERW is known to sca- venge ROS and protect DNA from oxidative damage. We previously showed that ERW was capable of extending lifespan of
by scaven-ging ROS . Molecular hydrogen could scavenge ROSand protected brain from oxidative stress . Pt NPs arealso a new type of multi-functional ROS scavenger .
Materials and methods
In this research, we used TI-200S ERW derived from 2mM NaOH solution produced by a batch type electroly-sis device and model waters containing molecularhydrogen and synthetic Pt NPs of 2-3 nm sizes asresearch models of ERW to examine the anti-oxidantcapabilities of ERW on several kinds of neural cells suchas PC12, N1E115, and serum free mouse embryo(SFME) cells. We pretreated the ERW and 200
and examined the neuroprotective effects of ERW on PC12, N1E115 and SFME cells, using WST-8method. We also examined the intracellular ROSscavenging effects of ERW on N1E115 cells after pre-treated cells with ERW and H
using DCFH-DA. Wechecked the protective effects of ERW on mitochondriaand cytoplasm by Rh123 and Fuo-3 AM stain. We alsoexamined the ATP production of SFME cells after pre-treated with ERW and H
by Bioluminescence Assay Kit. Finally, we used dissolved hydrogen (DH) and PtNPs as research models to examine their neuroprotec-tive effects.
ERW significantly reduced the cell death induced by H
pretreatment (Figure1). ERW also scavenged theintracellular ROS and prevented the decrease of mito-chondrial membrane potential and ATP productioninduced by ROS. We also examined the neuroprotectiveeffects of molecular hydrogen and Pt NPs and showedthat both molecular hydrogen and Pt NPs contributedto the neuroprotective effects of ERW.
The results suggest that ERW is beneficial for the pre- vention and alleviation of oxidative stress-inducedhuman neurodegenerative diseases.
Graduate School of Systems Life Sciences, Kyushu University, 6-10-1Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Department of Bioscienceand Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
Graduate School of Systems Life Sciences, Kyushu University, 6-10-1Hakozaki, Higashi-ku, Fukuoka 812-8581, JapanFull list of author information is available at the end of the article
© 2011 Yan et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative CommonsAttribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction inany medium, provided the original work is properly cited.