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GATE HELPLINE Bioprocess Engineering MCQ III

GATE HELPLINE Bioprocess Engineering MCQ III

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Published by Santhosh Kalash
This is GATE BIOTECH mock test paper for Bioprocess engineering.
This is GATE BIOTECH mock test paper for Bioprocess engineering.

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Published by: Santhosh Kalash on Apr 26, 2013
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08/26/2013

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This is copyrighted product of GATEHELPLINE
GATEHELPLINE
BIOPROCESSENGINEERINGMCQs-III
 
This is copyrighted product of GATEHELPLINE21.
 
Measurement of K
L
a in a bioreactor can be carried out by sodium sulfite oxidation method thatis based on the oxidation of sodium sulfite solution containing 0.003 M Cu
++
ions and the air wassparged in. After 10 minute the air flow was stopped and a 10 ml sample was taken and titrated.The concentration of the sodium sulfate in the sample was found to be 0.20 M. the oxygenuptake rate for this aerated system will work out to be:a)
 
0.08 g l
-1
s
-1
c) 0.8 g l
-1
s
-1
b)
 
0.008 g l
-1
s
-1
d) none of the above22.
 
Examine the date given below in the table on purification of a protein XStep Volume Protein Enzyme activityCrude cell free extract 500 12.0 5.0Ammonium sulfate precipitation 125 3.0 11.0Ion exchange chromatography 10 9.0 75.0The yield percent and purification factor respectively at the end of the experiment will be approximatelya)
 
25 and 3 c) 20 and 30b)
 
75 and 30
d) 30 and 20
23.
 
Which one of the following reactions is used for the purpose of recycling enzymes inbioprocesses?a)
 
Isomerization c) Phosphorylationb)
 
Polymerization
d) Immobilization
 24.
 
To optimize the bioreactor system, which one of the following conditions is least important foranaerobic fermentation?
a)
 
Culture agitation to maintain oxygen supply
b)
 
Restriction of the entry of contaminating organismsc)
 
Control of parameters like pH and temperatured)
 
Maintenance of constant culture volume25.
 
Power number, also Newton’s number. Is defined as a dimensionless parameter relating toa)
 
Turbulent flowb)
 
The relative velocity between the nutrient solution and individual cells
c)
 
Energy required by the stirred reactors
d)
 
None of the above26.
 
The selection of the appropriate purification method in the product recovery after microbialfermentation depends on thea)
 
Nature and the stability of the end products producedb)
 
Type of the side products presentc)
 
Degree of purification required
d)
 
All of the above
27.
 
Which of the following techniques is NOT ideal for immobilizing cell- free enzymes?a)
 
Physical entrapment by encapsulationb)
 
Covalent chemical bonding to surface carriers
c)
 
Physical bonding by flocculation
d)
 
Covalent chemical bonding by cross linking the precipitate
 
This is copyrighted product of GATEHELPLINE28.
 
If the fractional recovery at each step of unit operation is 0.8, the recovery after 4 step will bea)
 
0.24
c) 0.41
b)
 
3.23 d) 0.8229.
 
The factors likely to increase the rate of reaction catalyzed by a surface immobilized enzymeis/area)
 
Increased agitation of the bulk liquid containing the substrate
b)
 
Continued replacement of the bulk liquid containing the substrate
c)
 
Increased concentration of the substrate in the bulk liquidd)
 
All of the above30.
 
Which of the following cases are likely to lead to faster rates of catalysis by an enzymeimmobilized on a negatively charged support?
a)
 
A positively charged substrate and a negatively charged product
b)
 
A negatively charged substrate and a positively charged productc)
 
A positively charged substrate and a positively charged productd)
 
None of the above31.
 
Which of the following statements applies to the operation of a fed batch process?a)
 
The volume of the culture increases with timeb)
 
It helps controlling repressive effects of the nutrient being fedc)
 
It eliminates the need for oxygen supply
d)
 
Only (A) and (B) of the above
32.
 
In large scale fermentation, the preferred method of sterilization isa)
 
Chemicals c) radiationb)
 
Filtration
d) heat
 33.
 
In secondary metabolism two distinct – trophophase and idiophase refer respectively toa)
 
Growth and production phaseb)
 
Early and late phasec)
 
Primary and secondary metabolism
d)
 
Lag phase and log phase
34.
 
Which of the following is true for chemostat?
a)
 
Concentration of limiting substrate is maintained constant
b)
 
High productivity can not be achievedc)
 
Automation is difficultd)
 
Mutation is less likely to occur35.
 
For scaling up of a bioreactor, the following parameter is assumed to be constanta)
 
Airflow rateb)
 
Diameter of the impeller
c)
 
Agitator speed
d)
 
Volumetric mass transfer coefficient

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