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Table Of Contents

2.1 Structure and function of bone tissue
2.1.1 Bone matrix components
2.1.2 Bone cells
2.2 Cellular mechanisms of bone resorption
2.2.1 Osteoclastic membrane domains
2.2.2 Attachment of osteoclasts to bone matrix
2.2.3 Cadherins
2.2.4 Degradation of the mineral and organic matrix
2.2.5 Regulation of osteoclastic bone resorption
2.2.6 Disorders of bone modeling and remodeling
2.3 Cell polarity
2.4 Communication between bone cells
2.4.1 Gap junctions
4.1 Reagents (I-IV)
4.1.1 Antibodies (I-IV)
4.2 Cell culture (I-IV)
4.2.1 Isolation and culture of osteoclasts (I, III-IV)
4.2.2 Cell line (II)
4.2.3 Tissue culture (II)
4.3 Fluorescence and microscope techniques (I-IV)
4.3.1 Light microscopy (I, III-IV)
4.3.2 Conventional fluorescence microscopy (I, III-IV)
4.3.3 Confocal laser scanning microscopy (I-IV)
4.3.4 Immunoelectron microscopy (II)
4.3.5 Digital image analysis (I -IV)
4.4 Viral infections (II)
4.4.1 Metabolic labeling (II)
4.4.2 Cell surface biotinylation (II)
4.5 Statistical analysis (I, III-IV)
5.1 Cadherin localization in osteoclasts (I)
5.2 Effects of HAV-containing peptide (I)
5.2.1 Primary attachment of osteoclasts to bone surface
5.2.2 Osteoclastic bone resorption
5.2.3 Activity of osteoclasts
5.3 Polarity of osteoblasts (II)
5.3.1 Immunofluorescence data
5.3.2 Electron microscopy data
5.3.3 Biochemical data
5.4 Gap junctions in osteoclasts (III and IV)
5.4.1 Connexin-43 localization in osteoclasts (III)
5.4.2 Effects of gap-junctional inhibitors heptanol and Gap 27
6.1 Methodological aspects
6.2 Cadherins and attachment of osteoclasts to bone
6.3 Polarity of osteoblasts
6.4 Gap junctions in osteoclasts
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Attachment, Polarity

Attachment, Polarity

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Published by: AndykaYayanSetiawan on Apr 27, 2013
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02/06/2014

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