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Practical Biochemistry - Revision of Biochemistry

Practical Biochemistry - Revision of Biochemistry

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Published by Adlina Tajuddin

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Published by: Adlina Tajuddin on May 01, 2013
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05/07/2013

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REVISION OF BIOCHEMISTRY(GIT module)
(This power point is not originally fromsection of practical class. It is made toclarify all points that are emphasized byprofessors during revision class)*
do not depend on this power point only. Readthe topics inside the book is recommended.
 
ESTIMATION OF FREE AND TOTALACIDITY IN A GASTRIC JUICE SAMPLE
 
GASTRIC JUICE TITRATION
X1 X2
Definition of end point No of mls of 0.1 N NaOH inneutralizefreeacid in5mL gastric  juiceNo of mls of 0.1 N NaOH inneutralizetotalacid in5mL gastric  juiceAcidity: Free acidity (free HCl) Total Acidity (Free HCl + CombinedHCl (end point)+ organic acid)Indicator Topfer PhenolphthaleinpH 3 8No of mls of 0.1 N NaOH inneutralizefreeacid in100mL gastric juiceNo of mls of 0.1 N NaOH inneutralizetotalacid in100mL gastric juiceX1 x 100/5=X1 x 20
=………0.1N
NaOH %X2 x 100/5=X2 x 20
=………0.1N
NaOH %

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