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A. Background Photosynthesis is a process that only occurs in plants is chlorophyll and photosynthetic bacteria, where solar energy (in the form of photons) is captured and converted into chemical energy (ATP and NADPH). This chemical energy is used for photosynthesis of carbohydrates from water and carbon dioxide. So, all other organic molecules synthesized from plants of the energy and presence of other living organisms depend on plants or photosynthetic bacteria the ability to photosynthesize. Chlorophyll is a green photosynthetic pigment contained in plants, algae and Cynobacteria. name "chlorophyll" is derived from the ancient Greek: choloros = green (green), and phyllon = leaf (leaves). Krolofil function in plants is to absorb energy from sunlight to be used in the process of photosynthesis is a biochemical process by which plants synthesize carbohydrates (sugars into starch), of the gas carbon dioxide and water with the help of sunlight. Therefore conducted an experiment to determine the effect of light on photosynthesis speed. B. Purpose To determine the effect of light on photosynthesis speed. C. Benefit To understand the effect of light on photosynthesis speed in leaf discs.
B. Tools and Materials 1. 2 x 10 cm3 syringes 2. About 20 cm3 0,2 sodium hydrogen carbonate solution 3. Stop clock 4. Strong source of light 5. Cork borer no. 3 or jumbo straws 6. Plant e.g. ivy, clover, pelargonium (in spring and summer). Other plants are possible but they must be in good health and growing rapidly. It is best to check possible plants beforhand for this experiment. Watercress, for example, appears not to work. The plant do not need to be destarched. C. Work Procedure 1. Use a plastic straw to cut four leaf discs from a plant.
2. Remove the plunger from a 5 cm3 transparent plastic syringe. Add 5 cm3 of 0,2 M sodium hydrogen carbonate solution.
3. Gently blow the leaf discs out of the straw into the solution in the syringe.
6. Place a finger over the nozzle and gently pull the plunger down. Many bubbles will appear on the leaf discs.
7. Tap the syringe vigorously so that the air bubbles rise to the top. Repeat steps 5, 6, 7 until all discs sink. 8. Put it under the light-bank and start a stop watch. Record the time taken for each discs to rise.