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Extracted Pigment From Coffea arabica L. pericarp as a potential substitute in staining animal tissues

Extracted Pigment From Coffea arabica L. pericarp as a potential substitute in staining animal tissues

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Published by Erwin Pereña Peji
This is an undergraduate research about the production of non-hazardous and non-synthetic, inexpensive alternative stain from the utilization of the pericarp of fresh arabic coffee beans of Mendez, Cavite which are usually considered as residues of coffee processing. The research was performed at the Philippine Normal University Biology Laboratory.
This is an undergraduate research about the production of non-hazardous and non-synthetic, inexpensive alternative stain from the utilization of the pericarp of fresh arabic coffee beans of Mendez, Cavite which are usually considered as residues of coffee processing. The research was performed at the Philippine Normal University Biology Laboratory.

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Categories:Types, Research
Published by: Erwin Pereña Peji on May 03, 2013
Copyright:Attribution Non-commercial

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12/25/2013

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 Extracted Pigment from 
Coffea arabica L.
pericarp as aPotential Substitute in Staining Animal Tissues
An undergraduate research presented to theDepartment of Biological SciencesCollege of SciencePhilippine Normal UniversityIn Partial fulfilment of the courseBIOL13: Research in BiologySubmitted
 
to
 
Professor Genelita S. GarciaFaculty, Department of Biological SciencesCollege Of ScienceSubmitted byErwin P. PejiIV-A BSBTDecember 20, 2012
 
i ABSTRACT
The use and production of natural stains from plantsare very common nowadays due to the expensive commercially-synthesized stains. The purpose of this study was to assessthe potential of the ethanol extract of the pericarp offresh
Coffea arabica L.
in staining animal tissues. Thiswas performed by extracting pigment from the fresh pericarpof coffee beans through rotary evaporation using ethanol asthe solvent. The resulting extract was used to stain cross
sections of frog’s stomach, liver, skeletal muscle, small
intestine, and skin. Ten selected respondents observed andevaluated the physical appearance of the stained frogtissues using a rating scale instrument. Results shown thatin terms of the physical appearance of the stained tissues,stomach exhibited tissues that are stained in greatintensity and distinction in terms of clarity, non-granularin terms of uniformity, and parts mostly stained in termsof visibility. The data gathered were analysed using One-way ANOVA. Analysis shown that there is no significantdifference in the physical appearance of the frog tissuesstained with fresh coffee beans ethanol extract. For futureresearchers who will perform the same study, it is strongly
 
ii
recommended to make use of other solvents for extraction inorder to compare the quality of stain. Also, it isadvisable to use other specimens to be stained aside fromanimal tissues like bacteria, protists, fungi, and planttissues.

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