The use and production of natural stains from plantsare very common nowadays due to the expensive commercially-synthesized stains. The purpose of this study was to assessthe potential of the ethanol extract of the pericarp offresh
Coffea arabica L.
in staining animal tissues. Thiswas performed by extracting pigment from the fresh pericarpof coffee beans through rotary evaporation using ethanol asthe solvent. The resulting extract was used to stain cross
sections of frog’s stomach, liver, skeletal muscle, small
intestine, and skin. Ten selected respondents observed andevaluated the physical appearance of the stained frogtissues using a rating scale instrument. Results shown thatin terms of the physical appearance of the stained tissues,stomach exhibited tissues that are stained in greatintensity and distinction in terms of clarity, non-granularin terms of uniformity, and parts mostly stained in termsof visibility. The data gathered were analysed using One-way ANOVA. Analysis shown that there is no significantdifference in the physical appearance of the frog tissuesstained with fresh coffee beans ethanol extract. For futureresearchers who will perform the same study, it is strongly