PAPER 3 BIOLOGY SPM

1. An experiment was carried out to study the effect of amylase concentration on the rate of a biochemical reaction. Satu eksperimen telah dijalankan untuk mengkaji kesan amilase ke atas kadar tindakbalas biokimia. The following steps were carried out. Langkah-langkah berikut telah dijalankan. Step 1 Langkah 1 : Each test tube was filled with solutions as shown in Table 1. : Setiap tabung uji diisi dengan larutan seperti dalam Jadual 1. Volume of saliva (ml) Isipadu air liur (ml) 0.5 1.0 1.5 2.0 3.0 Table 1 Jadual 1 Step 2 Langkah 2 Step 3 Langkah 3 Step 4 : A few drops of iodine was dropped separately on a white tile. : Beberapa titis iodin dititiskan berasingan di atas jubin putih : 4 ml of starch solution was poured into each test tube. : 4ml larutan kanji dimasukkan ke dalam setiap tabung uji : The stopwatch was started. A dropper was used to withdraw a small amount of the mixture from the test tube. It is then added to the drop of iodine on the tile immediately. The change in colour was observed at an interval of 10 seconds until there was no change in colour in the iodine solution. : Jam randik dimulakan. Penitis digunakan untuk mengeluarkan sedikit campuran dari tabung uji. Campuran tadi dititiskan ke atas titisan iodin pada jubin putih dengan segera. Perubahan warna diperhatikan untuk setiap sela 10 saat sehingga tiada lagi perubahan warna berlaku ke atas larutan iodin. Volume of distilled water (ml) Isipadu air suling (ml) 2.5 2.0 1.5 1.0 0.0

Test tube Tabung uji A B C D E

Langkah 4

Diagram 1 (a), 1 (b) and 1 (c) show the apparatus set-up used in this experiment. Rajah 1 (a), 1 (b) dan 1 (c) menunjukkan susunan radas yang digunakan dalam eksperimen ini.

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5 1 ml saliva solution 1 ml larutan air liur

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Test tube Tabung uji

1% starch solution 1% larutan kanji

Diagram 1 (a) Rajah 1 (a)

Water bath at 37 C Kukus air pada 37 C

Dropper with iodine Penitis dengan iodin

White tiles with grooves Jubin putih berlekuk

Diagram 1 (b) Rajah 1 (b)

Hr : Min : Sec
Jam : Min : Saat

Stopwatch Jam randik

03 : 08 : 57

Diagram 1 (c) Rajah 1 (c)

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Table 2 shows the results of this experiment. Jadual 2 menunjukkan keputusan eksperimen ini.

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Test tube Tabung uji

Stopwatch reading Bacaan jam randik

Time taken when no change in colour in the iodine solution (minute) Masa yang diambil bila tiada lagi perubahan warna di dalam larutan iodin (minit)

A B C D E

00:09:00 00:06:00 00:04:00 00:02:00 00:01:80

Table 2 Jadual 2

(a) Record the time taken when there is no change in colour takes place in the iodine in the boxes provided in Table 2. Rekod masa yang diambil apabila tiada lagi perubahan warna pada larutan iodin di dalam Jadual 2. [3 marks] [3 markah]

1(a)

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(b) (i) State two different observations made from Table 2. Nyatakan dua pemerhatian yang berbeza yang dibuat daripada Jadual 2. Observation 1 / Pemerhatian 1 : Observation 2/ Pemerhatian 2 : [3 marks] [3 markah] (ii) State the inference which corresponds to the observation in (b)(i). Nyatakan inferens yang sepadan dengan pemerhatian di (b)(i). Inference from observation 1 / Inferens daripada pemerhatian 1:

1(b)(i)

1(b)(ii)

Inference from observation 2 / Inferens daripada pemerhatian 2:

[3 marks] [3 markah] (c) Complete Table 3 based on this experiment. Lengkapkan Jadual 3 berdasarkan eksperimen ini. Variable Pembolehubah Manipulated variable Pembolehubah dimanipulasikan Responding variable Pembolehubah bergerak balas Method to handle the variable Cara mengendali pembolehubah

. . .

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Constant variable Pembolehubah dimalarkan

. .

. . .

1(c)

Table 3 / Jadual 3

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(d) State the hypothesis for this experiment. Nyatakan hipotesis bagi eksperimen ini.

[3 marks] [3 markah]

....

1(d)
[3 marks] [3 markah]

(e) (i) Construct a table and record all the data collected in this experiment. Bina satu jadual dan rekodkan semua data yang dikumpul dalam eksperimen ini. Your table should have the following titles: Jadual anda hendaklah mengandungi tajuk-tajuk berikut: i. Test tube Tabung uji

ii. Enzyme concentration (%) Kepekatan Enzim (%) iii. Rate of reaction Kadar tindak balas

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Use the formula: Enzyme concentration =

Volume of saliva Total volume of solution

x 100%

Rate of reaction =

1 Time taken when no change in colour occurs in iodine

Gunakan formula: Kepekatan enzim =

Isipadu air liur Jumlah isipadu larutan 1

100%

Kadar tindakbalas =

masa diambil sehingga tiada lagi perubahan warna pada larutan iodin

1(e)(i)

[3 marks] [3 markah]

1(e)(ii ) 3

(e) (ii) Use the graph paper provided to answer this part of the question. Using the data in (e) (i), draw the graph of rate of reaction against the enzyme concentration. Guna kertas graf yang disediakan untuk menjawab ceraian soalan ini. Menggunakan data di (e)(i), lukis graf kadar tindakbalas melawan kepekatan enzim. [3 marks] [3 markah]

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(f) Based on the graph in (e)(ii), explain the relationship between the rate of reaction and the amylase concentration. Berdasarkan graf (e)(ii), terangkan hubungan antara kadar tindakbalas dan kepekatan amilase.

1(f) 3

.... ... [3 marks] [3 markah] (g) This experiment is repeated using a higher concentration of amylase. Predict the rate of reaction. Explain your prediction. Eksperimen ini diulangi dengan menggunakan kepekatan amilase yang lebih tinggi. Ramalkan kadar tindakbalas. Terangkan ramalan anda.

1(g)

.. ....

... [3 marks] [3 markah]

(i) In Table 4, list all the materials and apparatus labeled in Diagram 1 (a), 1 (b) and 1 (c). Dalam Jadual 4, senaraikan semua bahan dan radas yang berlabel dalam Rajah 1 (a), 1 (b) dan 1 (c). Material Bahan Apparatus Radas

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Table 4

( 3 marks

1(i)

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