HYPOTHESIS JUSTIFICATION
Light can have a dramatic effect on the growth of a plant. Light is used by plants during photosynthesis with the help of water (H
2
O) and carbon dioxide (CO
2
) under the influence of chlorophyll to produce glucose (C
6
H
12
O
6
), oxygen (O
2
) and residual water. The equation for photosynthesis can be written as:
Chlorophyll
6 CO
2
+ 12 H
2
O + light energy -----------
à
6 O
2
+ C
6
H
12
O
6
+ 6 H
2
OPhotosynthesis occurs in the thylakoid membrane of a chloroplast specifically using chlorophylland is the process by which light energy is converted into chemical energy which is then storedin the bonds of the glucose produced (J. Stein Carter, 2004). Photosynthesis occurs in 2 stages:the light dependant stage and the light independent stage. The light dependant reactions occur inthe first stage. In this phase chlorophyll traps light energy and uses it to produce ATP (adenosinetri-phosphate) molecules and split water into hydrogen ions and oxygen gas. Chlorophyll has amolecular structure that, when excited by photons of light energy, initiates a series of steps thatresult in the production of ATP molecules (Heinemann Education, 2004). The equation for thesereactions could be written as:
Chlorophyll
Water ------------
à
hydrogen ions + oxygen gas + ATP
Light energy
The second stage of photosynthesis is independent of light. ATP made during the first stage provides the energy needed to combine CO
2
with the hydrogen ions which are also from the lightdependant reactions. These reactions form glucose, a molecule rich in energy and some water isreproduced. The equation for the light independent reactions can be writer as:ATP + H
+
ions + CO
2
à
glucose + water + ADP (adenosine di-phosphate)(Heinemann Education, 2004).For photosynthesis to occur, this light energy
must
be provided to provide the energy needed for all the reactions to occur. Not all wavelengths of light can support photosynthesis however. The spectrum range that photosynthesis works in depends on the accessory pigments present in the plant. For example, in green plants the absorption spectrums for chlorophyll andcarotenoids (these also play a part in photosynthesis)which peak for purple-blue and red light resemble theaction spectrum, which shows which wavelengths of light are most effective in a specific chemicalreaction. (Wikipedia, 2008). Chlorophyll which isabundant in green plants appears green because itreflects green light. This green light can not beabsorbed and thus it cannot be used in photosynthesisin green plants. However, red and blue light areabsorbed by chlorophyll and it is the energy fromthese colours that is used during photosynthesis (J.Stein Carter, 2004). The non-absorbed part of thelight spectrum is what gives photosynthetic organismstheir colour and
Figure 2.
The light spectrum and the rateof photosynthesis (Wikipedia 2008).
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