Nurul Wiqoyah

Klasifikasi dan identifikasi virus

Awal 1900an, virus dikelompokkan tersendiri Identifikasi:

Karakteristik biologi, morfologi, struktur genom, sifat antigenik

Morfologi: ukuran, bentuk, capsid symmetry, ada tidaknya envelop Sifat fisik virion, struktur genom lipid pattern, KH, protein struktural dan non struktural,\ sifat antigenik dan biologi , strategi replikasi , host range, mode of

transmission dan patogenitas

 Klasifikasi Ordo Famili Subfamili

Genera
Species

---- virales ---- viridae ---- virinae ---- virus

Taxonomy Ordo Mononegairales

Order Mononegavirales Family Bornaviridae Bornavirus Rhabdoviridae Vesiculovirus Lyssavirus Ephemerovirus Novirhabdovirus Cytorhabdovirus Nucleorhabdovirus Filoviridae Marburghvirus Ebolavirus Paramyxoviridae Paramyxovirinae Rubulavirus Avulavirus Respirovirus Henipavirus Morbillivirus Pneumovirinae Pneumovirus Metapneumovirus Human respiratory syncytial virus Avian metapneumovirus V V Mumps virus Newcastle disease virus Sendai virus Hendra virus Measles virus V V V V V Lake Victoria marburgvirus Zaire ebolavirus V V Vesicular stomatitis Indiana virus Rabies virus Bovine ephemeral fever virus Infectious hematopoietic necrosis virus Lettuce necrotic yellows virus Potato yellow dwarf virus V, I V V, I V P, I P, I Borna disease virus V Subfamily Genus Type Species Hosta

V, vertebrate; I, insect; P, plant. Fields Vriology (2007) 5th edition, Knipe, DM & Howley, PM, eds, Wolters Kluwer/Lippincott Williams & Wilkins, Philadelphia Table 2.2

Klasifikasi virus manusia

"Group" dsDNA Family Poxviridae Herpesviridae Adenoviridae Polyomaviridae Papillomaviridae ssDNA Anellovirus Parvoviradae Retro Hepadnaviridae Retroviridae dsRNA Reoviridae ssRNA (-) Rhabdoviridae Filoviridae Paramyxoviridae Orthomyxoviridae Bunyaviridae Arenaviridae Deltavirus ssRNA (+) Picornaviridae Calciviridae Hepevirus Astroviridae Coronaviridae Flaviviridae Togaviridae ssRNA (+) ssRNA (+) ssRNA (+) ssRNA (+) ssRNA (+) ssRNA (+) ssRNA (+) 7 to 9 7 to 8 7 6 to 7 28 to 31 10 to 12 11 to 12 Icosahedral Icosahedral Icosahedral Isometric Helical Spherical Icosahedral No No No No Yes Yes Yes ssRNA (-) ssRNA (-) ssRNA (-) ssRNA (-), segmented ssRNA (-, ambi), segmented ssRNA (-, ambi), segmented ssRNA (-) circular 11 to 15 19 10 to 15 10 to 13.6 11 to 19 11 2 Helical Helical Helical Helical Helical Circular, nucleosomal Spherical Yes Yes Yes Yes Yes Yes Yes dsRNA, segmented 19 to 32 Icosahedral No dsDNA (partial), circular ssRNA (+), diploid 3 to 4 7 to 13 Icosahedral Spherical, rod or cone shaped Yes Yes ssDNA circular ssDNA, linear, (- or +/-) 3 to 4 5 Isometric Icosahedral No No Genome dsDNA, linear dsDNA, linear dsDNA, linear dsDNA, circular dsDNA, circular Genome size (kb) Capsid 130 to 375 125 to 240 26 to 45 5 7 to 8 Ovoid Icosahedral Icosahedral Icosahedral Icosahedral Envelope Yes Yes No No No

Fields Vriology (2007) 5th edition, Knipe, DM & Howley, PM, eds, Wolters Kluwer/Lippincott Williams & Wilkins, Philadelphia Table 2.1

Struktur dasar virus

DNA/ RNA + Capsid protein ---Nucleocapsid ---

Naked virus
Nucleocapsid + lipid membran, glicoprotein ---

enveloped virus

Brock Biology of Microorganism, 2012, M.T. Madigan et al

Icosahedral Cymmetry

Icosahedral

A, Human papilloma Virus (HPV), B. Tiga dimensi HPV

Helix

Helical Naked Capsid Virus

Bacteriophage

Virology, principle and Application, 2007, John Carter, V, Saunders

Replikasi virus
4 fase dasar:
Attachment Entry/ Penetration Genome uncoating Genome replication and protein synthesis Assembling progeny Exit/ release

Attachment dan entry

Virology, principle and Application, 2007, John Carter, V, Saunders

Reseptor sel, protein virus dalam attachment

Virus

Reseptor sel

Protein yang terlibat dalam Attachment ke reseptor Fusi

Naked virus 90% Rhinovirus mns Poliovirus HIV-1 Virus influenzae A,B

Intercellular mol adhesion 1 (ICAM 1) CD155 CD4

VP1 +VP3

VP1 Gp120 Gp41 Haemagglutinin

Glycoprotein yang Haemagglutinin mengandung sialic acid

Genome uncoating
Tergantung Virus, proses dapat terjadi: 1. pada permukaan sel, capsid tetap di permukaan sel 2. dalam sitoplasma 3. dalam pori nukleus 4. dalam nukleus

Assembly dan release

Virology, principle and Application, 2007, John Carter, V, Saunders