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BIMM 110 Section 4 Pset

BIMM 110 Section 4 Pset

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Published by George Chen
BIMM 110 Molecular Basis of Human Disease Problem Set #4 by George Chen
BIMM 110 Molecular Basis of Human Disease Problem Set #4 by George Chen

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Categories:Types, School Work
Published by: George Chen on Apr 24, 2009
Copyright:Attribution Non-commercial


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BIMM 110 Molecular Basis of Human Disease
 TA: George Chengtchen@ucsd.eduOH: Tu 12:30-1:30 Sierra Summit or by appointment (See below)Section: W 4:00-4:50 Center 203
Problem Set 4
•Office hours this week: 12:45-1:45 UCSD Guardian office - 2
floor, OldStudent Center, above Groundworks Books•No answers for problem sets will be posted, we will go over the problems insection•The following problems cover lectures 7 & 8•Section on May 6
(day after midterm) is
Please check WebCT for an announcement regarding midterm roomassignments
Good luck on the midterm!
1. You know that Chronic Mylenogenous Leukemia is the result of the fusionprotein BCR-ABL. You wish to examine the expression level of the promoterthat encodes for this fusion protein. Design an experiment that could be usedto determine the promoter's expression level.2. You believe that you have found a gene BIMM which may be a proto-oncogene, that is, a gene that when mutated, becomes an oncogene (cancercell). You aim to test this theory by knocking in the mutant gene in a mouse.Explain the steps to accomplish this at a molecular level.3. What is the importance of using two drugs in selecting for knockedout/knocked in genes in recombinant embryos?
4. A researcher wants to test her genotype for X-inactivation skewing usingGFP. How may she accomplish this?5. Some scientists consider cell lines not a truly valid way for studying truecancer cell responses in vivo. Why not?6. What is the advantage of using the LoxP-Cre protocol to knock out a genein mice?7. Scientists discover a new protein FISH which apparently causes ahydration-related disease when overexpressed. It appears to be an inheriteddisease. You are looking to find where FISH is coded in the human genome.How would you do this? What kind of protocol is this?8. You want to create a genetic map for two populations of mice that youhave bred. Assume that fur color and tail length are controlled by singlegenes. They show autosomal dominant inheritance. Observe the followingdata:P10 black fur, long tail mice x10 white fur, short tail miceF125 black fur, long tail micex25 white fur, short tail

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