BIMM 110 Molecular Basis of Human Disease
TA: George Chengtchen@ucsd.eduOH: Tu 12:30-1:30 Sierra Summit or by appointment (See below)Section: W 4:00-4:50 Center 203
Problem Set 4
•Office hours this week: 12:45-1:45 UCSD Guardian office - 2
floor, OldStudent Center, above Groundworks Books•No answers for problem sets will be posted, we will go over the problems insection•The following problems cover lectures 7 & 8•Section on May 6
(day after midterm) is
Please check WebCT for an announcement regarding midterm roomassignments
Good luck on the midterm!
1. You know that Chronic Mylenogenous Leukemia is the result of the fusionprotein BCR-ABL. You wish to examine the expression level of the promoterthat encodes for this fusion protein. Design an experiment that could be usedto determine the promoter's expression level.2. You believe that you have found a gene BIMM which may be a proto-oncogene, that is, a gene that when mutated, becomes an oncogene (cancercell). You aim to test this theory by knocking in the mutant gene in a mouse.Explain the steps to accomplish this at a molecular level.3. What is the importance of using two drugs in selecting for knockedout/knocked in genes in recombinant embryos?