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Table Of Contents

1. FUNCTIONS OF EPITHELIUM
2. HISTOLOGY
3. EMBRYOLOGICAL ORIGIN
4. STEM CELLS AND MATURATION
5. ISOLATION AND CULTURE
5.1. Disaggregation
5.2. Selective Attachment
5.3. Selective Detachment
5.4. Substrate Modification
5.5. Feeder Layers
5.6. Cell Separation
5.7. Selective Culture
6. CHARACTERIZATION
7. CROSS-CONTAMINATION
8. DIFFERENTIATION
8.1. Induction of Differentiation
9. TRANSFORMATION
REFERENCES
APPENDIX: SOURCES OF MATERIALS
E. Kenneth Parkinson and W.AndrewYeudall
Joyce Taylor-Papadimitriou2
Margaret A. Stanley
Donna M. Peehl
Roland C. Grafstro¨m
Christiane Guguen-Guillouzo
Ludwik K.Trejdosiewicz3
Index
P. 1
Culture of Epithelial Cells

Culture of Epithelial Cells

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Published by Wiley
"...a wonderful compendium of current in vitro approaches that will be a useful resource to those just starting to work with an epithelial cell system as well as those that have been working with them for years and years."
Pharmaceutical Research

This completely revised and expanded new edition provides detailed descriptions of fundamental and practical aspects relating to the in vitro cultivation of disparate types of epithelia. In recent years, the use of epithelial cell culture in cell biology and tissue engineering has increased dramatically. This revision reflects those advances by including new chapters on the culture of animal and human hepatocytes, kidney epithelium, and bladder epithelium. Each chapter provides an introductory review of the principles and advantages of the particular method, followed by detailed protocols, practical tips, alternate methods, and a useful list of materials and suppliers.

"...a wonderful compendium of current in vitro approaches that will be a useful resource to those just starting to work with an epithelial cell system as well as those that have been working with them for years and years."
Pharmaceutical Research

This completely revised and expanded new edition provides detailed descriptions of fundamental and practical aspects relating to the in vitro cultivation of disparate types of epithelia. In recent years, the use of epithelial cell culture in cell biology and tissue engineering has increased dramatically. This revision reflects those advances by including new chapters on the culture of animal and human hepatocytes, kidney epithelium, and bladder epithelium. Each chapter provides an introductory review of the principles and advantages of the particular method, followed by detailed protocols, practical tips, alternate methods, and a useful list of materials and suppliers.

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Publish date: Apr 7, 2004
Added to Scribd: Jun 06, 2013
Copyright:Traditional Copyright: All rights reservedISBN:9780471459125
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