Tabel 1 Pengukuran kurva standar TMP

Konsentrasi (m) Blanko 0.5 2.5 5 7.5 10 15 Contoh perhitungan : Volume pengenceran TMP 15 m V1 x N1 = V2 x N2 Absorbansi 0 0.087 0.483 1.106 1.750 1.693 2.798

V1 x 30 m = 20 ml x 15 m V1 = = 10 m

Volume pengenceran TMP 10 m V1 x N1 = V2 x N2

V1 x 15 m = 20 ml x 10 m V1 = = 13.3 m

Volume pengenceran TMP 7.5 m V1 x N1 = V1 x N2

V1 x 10 m = 20 ml x 7.5 m V1 = = 15 m

Volume pengenceran TMP 5 m V1 x N1 = V2 x N2

V1 x 7.5 m = 20 ml x 5 m V1 = = 13.3 m

Volume pengenceran TMP 2.5 m V1 x N1 = V1 x N2

V1 x 5 m = 20 ml x 2.5 m V1 = = 10 m

Volume pengenceran TMP 1 m V1 x N1 = V1 x N2

V1 x 2.5 m = 20 ml x 1 m

V1

= 8 m

Volume pengenceran TMP 0.5 m V1 x N1 = V1 x N2

V1 x 1 m = 20 ml x 0.5 m V1 = = 10 m

3 2.5 Absorbansi 2 1.5 1 0.5 0 0 5 10 15 20 konsentrasi (m) y = 0.185x + 0.0609 R = 0.9738

Gambar 1 Kurva standar TMP