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The Cytoskeletonand Cell Motility
Cell and Molecular BiologyLecture 9
John Donnie A. Ramos, Ph.D.
Department of Biological SciencesCollege of ScienceUniversity of Santo Tomas
Cytoskeleton
The “skeletal system”of cells
Composed of filamentous structures
Microtubules –rigid tubes
tubulin
Microfilaments –solid, thin structures
actin
Intermediate filaments –tough, ropelike fibers
keratin, vimentin, desmin, Laminetc.
Highly dynamic structures
 
Functions of the Cytoskeleton
Microtubules and Peroxisomes
Peroxisomesare labeled with GFP while microtubules are labeledwith antibodies fused with a red fluorescent dye.
 
Studying the Cytoskeleton
Fluorescence microscopy
Real-time study
Ideal for studying the dynamics of cytoskeleton
Uses antibodies and fluorescent dyes/proteins
Identifying the location proteins in small amounts
centrin
Studying the Cytoskeleton
 Video microscopy
Monitoring of cell movement (real-time)
In vitro motility assay
Used to study motor proteins
Made possible with the development of nanotechnology
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