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Exp 3.8 (Form 4)

Exp 3.8 (Form 4)

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Published by IMELDA
Studying the effects of hypotonic, hypertonic and isotonic solutions on plant cells.
Studying the effects of hypotonic, hypertonic and isotonic solutions on plant cells.

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Published by: IMELDA on Jul 06, 2013
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01/25/2014

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3.8 Studying the effects of hypotonic, hypertonic and isotonicsolutions on plant cells
Problem statement:What are the effects of hypotonic, hypertonic and isotonic solutions on plantcells?Hypothesis:Hypotonic solution causes the cell to become swells and turgid, hypertonicsolution causes the cell become shrink and plasmolyse while isotonic solutioncauses the cell has no change.Variables:Manipulative variable – Types of solutionResponding variable – Shape of the cellConstant variable – Types of cellMaterials and apparatus:An onion bulb, 0.15 M and 0.50 M sodium chloride solutions, distilled water,filter paper, microscope slides, cover slips, a pair of forceps, mountingneedle and a light microscope.Procedures:1.Prepare four slides and label them A, B, C and D. (P1)2.Peel off an epidermal layer (skin) from the inner surface of an onionscale leaf using a pair of forceps. (P1)3.Mount the epidermis in a drop of distilled water on slide A. Lower acover slip slowly to avoid air bubbles from being trapped under thecover slip. (P1, P5)4.Examine the slide under a light microscope. Draw and label theepidermal. (P3)5.Place a drop of 0.15 M sodium chloride solution at one side of the coverslip. Allow the solution to spread beneath the cover slip by placing filterpaper on the other side of the cover slip. This is slide B. (P1, P2, P5)6.Observe the epidermal cells under the microscope. Draw and label theepidermal cells. (P3)7.Repeat steps 2 to 6 by substituting 0.5 M sucrose solution with 1.0 Msucrose solution. This is slide C. (P4)8.Transfer the epidermal cells that were soaked in 1.0 M sucrose solutioninto a drop of distilled water. This is slide D. (P4)9.Observe the epidermal cell under the microscope. Draw and label theepidermal cells. (P3)
©Imelda anak Nyaun/S.M.K. Serian
 
Results:
SlideTypes of solutionShape of plant cells
APlant cells in distilledwaterBPlant cells in 0.5 Msucrose solutionCPlant cells in 1.0 Msucrose solutionDPlant cells that aretransferred from the 1.0M sucrose solution intodistilled water
©Imelda anak Nyaun/S.M.K. Serian

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