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H202 SOP 07.11.13

H202 SOP 07.11.13

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Published by 420leaks
Analytical 360 Standard Operating Procedure
Hydrogen Peroxide Use In Cannabis Cultivation
Cannabis Action Coalition research project
Analytical 360 Standard Operating Procedure
Hydrogen Peroxide Use In Cannabis Cultivation
Cannabis Action Coalition research project

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Published by: 420leaks on Jul 14, 2013
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09/02/2013

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CONFIDENTIAL
Analytical 360 Standard Operating Procedure
Procedure No:1
Title:
Hydrogen Peroxide Use In Cannabis Cultivation
Version No:Issue date:17-09-13Page:
1
of 
5
Edward StremlowRandall Oliver07/09/13
Prepared byApproved ByReview Date
1.0PURPOSE
This document describes how to perform the collection and microbial analysis of flower samples at the Analytical 360 lab. Analytical 360 will analyze microbiallevels (total aerobic plate count and yeast & mold plate count) and absence of Salmonella and E. coli, in cannabis samples which have been treated with H202immediately after cultivation. Samples will also be analyzed prior to, and duringeach of the drying and curing stages of the flower; to determine effectiveness inreducing pathogens in H202 washed cannabis as compared to untreated flowers.“Aerobic plate count and yeast & mold plate count. The USP microbial limit test isperformed at Analytical 360 and is used to estimate the total number of viablemicro-organisms present and for the absence of specific pathogens in productsof all kinds. The microbial limit test is based on each viable microbe presentforming a colony. The number of the colony-forming units, CFU on the plate per gram determines the acceptability of the product. Microbial Counts (USP 2012)provide meaningful information regarding the microbial acceptability of products.”Hydrogen Peroxide is used for oxidation, bleaching, sterilization and as adisinfectant in a variety of industries. In recent years, many new agriculturalapplications for hydrogen peroxide have emerged as an effective measure for combating pathogens in livestock, fish aquaculture, and is also used as asterilization agent which is sprayed on crops. Studies have also shown H202treatment in water helps promote root and vegetative growth, improving cropyields, and reduce fungal diseases.The purpose of this study is to determine whether H202 treatment is a viablemeans of reducing fungal diseases and improve crop viability after cultivation.The scope of this project is not intended to analyze possible beneficialapplications of H202 during the growing stage, and is only intended to analyzeeffectiveness after cultivation. Cannabinoid profiling will also be analyzed todetermine whether H202 treatment will compromise quality or potency.
2.0SCOPE
This SOP applies to all cultivators donating samples and lab techniciansperforming microbial analysis of flower samples.
3.0RESPONSIBILITY
Donation, Collection and Delivery of Samples - CultivatorsMicrobial Analysis -This procedure must be performed by Analytical 360 LabTechnician.Organizing Cultivators – Cannabis Action Coalition
 
CONFIDENTIAL
Analytical 360 Standard Operating Procedure
Procedure No:1
Title:
Hydrogen Peroxide Use In Cannabis Cultivation
Version No:Issue date:17-09-13Page:
2
of 
5
Edward StremlowRandall Oliver07/09/13
Prepared byApproved ByReview Date
4.0MATERIALS
Cultivators – Sterol, sealed Bag for transportation of samples, Cannabis SamplesCannabis Action Coalition – 35% Food Grade Hydrogen Peroxide, Water, Two 5-10 gallon Holding/Wash tubs, Peroxide test strips. Analytical 360 - TSA plate (trypticase soy agar), Sab Dex plate (Sabourauddextrose agar),
 
2 mortar & pestles, 2 foil pieces approx. 3” x 3”, scoop, tweezers,70% isopropyl alcohol (IPA), beaker, HEPA mask, gloves, permanent marker 
5.0EQUIPMENT
Cannabis Action Coalition – Holding/Washing Tubs, Peroxide test strips. Analytical 360 - incubator (See SOP## for incubator operating instructions)Biohazard boxScale (See SOP## for scale operating instructions)Torch
6.0PROCEDURES:Cultivators:
1.Remove 3 branches or stems from each plant, containing either 1 large flower, or several small to medium size flowers. Leave all foliage on branch.2.Place samples in a large sterol, and sealable bag to prepare for transportation.Take caution to limit or avoid contamination.3.Samples collected from different plants need to be separated in separate bags.
4.
Deliver to Analytical 360, Tuesday, July 16
th
between 10AM – 5PM.
Cannabis Action Coalition:
5.Coordinate with a maximum of 6 cultivators for the sample collection and deliveryof 6 different cannabis strains.6.Oversee the collection of supplies and assist with H202 treatment and cleanup.7.Prepare 2, 5-10 gallon washing tubs. 1 tub treated with H202 for soak, and 1 tubwith untreated water for rinse.8.Mix 1/2 pint of 35% food grade H202 per 10 gallons of water.9.Prepare untreated water for rinse.10.Soak plants for 3-5 minutes in treated H202 and rinse following treatment.11.Hang Plants for drying and prepare for transportation, for drying and curingoffsite.
 
CONFIDENTIAL
Analytical 360 Standard Operating Procedure
Procedure No:1
Title:
Hydrogen Peroxide Use In Cannabis Cultivation
Version No:Issue date:17-09-13Page:
3
of 
5
Edward StremlowRandall Oliver07/09/13
Prepared byApproved ByReview Date
ANALYTICAL 360:
1.Collect all samples required for initial analysis and equipment necessary2.Put on lab coat3.Wash hands4.Put on gloves
Change gloves anytime you touch something that has not been disinfected.
5.Disinfect the torch, table surface, and other equipment with an IPA wipe6.Pour isopropyl alcohol into the beaker 2 cm deep and submerge implement tips(scoop, tweezers)7.With a permanent marker, label the bottom of the TSA plate and Sab Dex platewith sample's ANL number 8.Remove implements from IPA9.Close all IPA containers and place in designated area10.Make sure the IPA has dried from disinfected surfaces11.Start the torch and set on low12.Flame the tips of implements (scoop, tweezers)13.Make 2 foil boats14.Weigh foil boat A and tare scale15.Using tweezers, transfer herb from container to foil boat16.Weigh 0.1 g herb17.Using IPA in beaker, rinse mortar & pestle A18.Place IPA in designated area19.Flame the mortar & pestle20.Dump herb from foil boat into mortar 21.Grind herb in mortar & pestle22.Open the two control plates and set them at the back of your work area23.Using scoop, scrape herb away from the mortar sides and distribute evenly onthe bottom of the mortar. Perform dilutions in base and prepare samples for TSAplate.24.Quickly open TSA plate, upend mortar, and dispense solution into TSA plate.25.Quickly cover plate with its lid26.While the plate sits on the table, shake the plate a little back and forth todistribute herb across the agar 27.Weigh foil boat B and tare scale28.Using tweezers, transfer herb from customer's container to foil boat29.Weigh 0.1 g herb30.Rinse mortar & pestle B with IPA31.Flame the mortar & pestle32.Dump herb from foil boat B into mortar 33.Grind herb in mortar & pestle34.Using spatula scrape herb away from the mortar sides and distribute evenly onthe bottom35.Perform dilutions in base and prepare samples for Sab Dex.36.Quickly open Sab Dex plate, upend mortar, and dispense contents into plate37.Quickly cover plate with its lid

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